Click image to see more details
Product Info Summary
| SKU: | PB9816 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-RAP1A Antibody Picoband®
SKU/Catalog Number
PB9816
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RAP1A Antibody Picoband® catalog # PB9816. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RAP1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9816)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RAP1A, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9816 is reactive to RAP1A in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
21 kDa
Calculated molecular weight
20987 MW
Background of RAP1A
Ras-related protein Rap-1A is a protein that in humans is encoded by the RAP1A gene. This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. And it is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. Additionally, the encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9816 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Rat Liver Tissue, Rat Kidney Tissue, MCF-7 Whole Cell, SW620 Whole Cell, rat heart tissue, rat lung tissue, rat stomach tissue, rat kidney tissuemouse heart tissue, mouse lung tissue, mouse stomach tissue, mouse kidney tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RAP1A using anti-RAP1A antibody (PB9816).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Brain Tissue Lysate,
Lane 2: Rat Liver Tissue Lysate,
Lane 3: Rat Kidney Tissue Lysate,
Lane 4: MCF-7 Whole Cell Lysate,
Lane 5: SW620 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1A antigen affinity purified polyclonal antibody (Catalog # PB9816) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAP1A at approximately 21KD. The expected band size for RAP1A is at 21KD.
Click image to see more details
Figure 2. Western blot analysis of RAP1A using anti-RAP1A antibody (PB9816).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat stomach tissue lysates,
Lane 4: rat kidney tissue lysates.
Lane 5: mouse heart tissue lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse stomach tissue lysates,
Lane 8: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1A antigen affinity purified polyclonal antibody (Catalog # PB9816) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAP1A at approximately 21KD. The expected band size for RAP1A is at 21KD.
Protein Target Info & Infographic
Gene/Protein Information For RAP1A (Source: Uniprot.org, NCBI)
Gene Name
RAP1A
Full Name
Ras-related protein Rap-1A
Weight
20987 MW
Superfamily
small GTPase superfamily
Alternative Names
Ras-related protein Rap-1A;C21KG;G-22K;GTP-binding protein smg p21A;Ras-related protein Krev-1;RAP1A;KREV1; RAP1A C21KG, G-22K, KREV-1, KREV1, RAP1, SMGP21 RAP1A, member of RAS oncogene family ras-related protein Rap-1A|GTP-binding protein smg p21A|Ras-related protein Krev-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAP1A, check out the RAP1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAP1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAP1A Antibody Picoband® (PB9816)
Hello CJ!
No publications found for PB9816
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-RAP1A Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-RAP1A Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
2 Customer Q&As for Anti-RAP1A Antibody Picoband®
Question
We are currently using anti-RAP1A antibody PB9816 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
The anti-RAP1A antibody (PB9816) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-19
Question
What is the formulation for Anti-RAP1A Antibody Picoband?
Verified customer
Asked: 2019-11-11
Answer
The Anti-RAP1A Antibody Picoband contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Boster Scientific Support
Answered: 2019-11-11
