Anti-Prickle-like protein 2 PRICKLE2 Antibody

Product Name

Anti-Prickle-like protein 2 PRICKLE2 Antibody

View all PRICKLE2 Antibodies

SKU/Catalog Number

A08874

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Prickle-like protein 2 PRICKLE2 Antibody (Catalog # A08874). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

PRICKLE2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Prickle-like protein 2 PRICKLE2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08874)

Host

Rabbit

Contents

PRICKLE2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PRICKLE2 antibody was raised against a 15 amino acid synthetic peptide near the center of human PRICKLE2. The immunogen is located within amino acids 420 - 470 of PRICKLE2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Two isoforms of PRICKLE2 are known to exist. PRICKLE2 antibody is predicted to not cross-react with other PRICKLE protein family members.

Reactive Species

A08874 is reactive to PRICKLE2 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

95615 MW

Background of PRICKLE2

PRICKLE2, also known as Pk2 or EPM5, is a member of a highly conserved protein family that function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity. Both PRICKLE2 and the related protein PRICKLE1 are expressed in postmitotic neurons and promote neurite outgrowth, and both proteins promote neurite outgrowth via the Dishevelled dependent pathway in C1300 cells. PRICKLE2 localizes to the postsynaptic density and interacts with PSD-95 and NMDA receptors. Defects in the gene encoding PRICKLE2 are associated with autosomal recessive progressive myoclonic epilepsy.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08874 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PRICKLE2 antibody can be used for detection of PRICKLE2 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For PRICKLE2 (Source: Uniprot.org, NCBI)

Alternative Names

EPM5, Prickle-like protein 2 PRICKLE2 EPM5 prickle planar cell polarity protein 2 prickle-like protein 2|prickle homolog 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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