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Product Info Summary
| SKU: | A08874 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Prickle-like protein 2 PRICKLE2 Antibody
SKU/Catalog Number
A08874
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Prickle-like protein 2 PRICKLE2 Antibody (Catalog # A08874). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PRICKLE2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Prickle-like protein 2 PRICKLE2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08874)
Host
Rabbit
Contents
PRICKLE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PRICKLE2 antibody was raised against a 15 amino acid synthetic peptide near the center of human PRICKLE2. The immunogen is located within amino acids 420 - 470 of PRICKLE2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Two isoforms of PRICKLE2 are known to exist. PRICKLE2 antibody is predicted to not cross-react with other PRICKLE protein family members.
Reactive Species
A08874 is reactive to PRICKLE2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
95615 MW
Background of PRICKLE2
PRICKLE2, also known as Pk2 or EPM5, is a member of a highly conserved protein family that function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity. Both PRICKLE2 and the related protein PRICKLE1 are expressed in postmitotic neurons and promote neurite outgrowth, and both proteins promote neurite outgrowth via the Dishevelled dependent pathway in C1300 cells. PRICKLE2 localizes to the postsynaptic density and interacts with PSD-95 and NMDA receptors. Defects in the gene encoding PRICKLE2 are associated with autosomal recessive progressive myoclonic epilepsy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08874 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PRICKLE2 antibody can be used for detection of PRICKLE2 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PRICKLE2 in A-20 lysate with PRICKLE2 antibody at 1 μg/mL.
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Immunofluorescence of PRICKLE2 in rat brain cells with PRICKLE2 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For PRICKLE2 (Source: Uniprot.org, NCBI)
Gene Name
PRICKLE2
Full Name
Prickle-like protein 2
Weight
95615 MW
Superfamily
prickle / espinas / testin family
Alternative Names
EPM5, Prickle-like protein 2 PRICKLE2 EPM5 prickle planar cell polarity protein 2 prickle-like protein 2|prickle homolog 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRICKLE2, check out the PRICKLE2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRICKLE2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prickle-like protein 2 PRICKLE2 Antibody (A08874)
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