Product Info Summary
SKU: | M02771 |
---|---|
Size: | 100 µl |
Reactive Species: | Dog, Human, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-RANGAP1 Mouse Monoclonal Antibody [Clone ID: OTI1B4]
SKU/Catalog Number
M02771
Size
100 µl
Description
Boster Bio Anti-RANGAP1 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4). Catalog# M02771. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RANGAP1 Mouse Monoclonal Antibody [Clone ID: OTI1B4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02771)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1B4
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human RANGAP1 (NP_002874) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02771 is reactive to RANGAP1 in Dog, Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
63.4 kDa
Calculated molecular weight
63.542kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02771 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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Flow cytometric Analysis of Jurkat cells
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HEK293T cells transfected with either RANGAP1 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RANGAP1 antibody (M02771)
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Flow cytometric Analysis of Hela cells
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Anti-RANGAP1 mouse monoclonal antibody (M02771) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RANGAP1.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RANGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RANGAP1.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-RANGAP1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RANGAP1 monoclonal antibody.
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Western blot analysis of extracts (10ug) from a mouse cell line by using anti-RANGAP1 monoclonal antibody (1:200).
Protein Target Info & Infographic
Gene/Protein Information For RANGAP1 (Source: Uniprot.org, NCBI)
Gene Name
RANGAP1
Full Name
Ran GTPase-activating protein 1
Weight
63.542kDa
Superfamily
RNA1 family
Alternative Names
Fug1; KIAA1835Fug1; MGC20266; Ran GTPase activating protein 1; ran GTPase-activating protein 1; RanGAP1; SD; segregation distorter homolog (Drosophila); segregation distorter homolog; segregation distortion RANGAP1 Fug1, RANGAP, SD Ran GTPase activating protein 1 ran GTPase-activating protein 1|segregation distorter homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RANGAP1, check out the RANGAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RANGAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RANGAP1 Mouse Monoclonal Antibody [Clone ID: OTI1B4] (M02771)
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