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Product Info Summary
| SKU: | A00981 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-RanBP2 Antibody Picoband®
SKU/Catalog Number
A00981
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RanBP2 Antibody Picoband® catalog # A00981. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RanBP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00981)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RanBP2, different from the related mouse sequence by nine amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00981 is reactive to RANBP2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
270 kDa
Calculated molecular weight
358199 MW
Background of RANBP2
RAN binding protein 2 (RANBP2) is protein which in humans is encoded by the RANBP2 gene. This gene encodes a very large RAN-binding protein that immunolocalizes to the nuclear pore complex. The protein is a giant scaffold and mosaic cyclophilin-related nucleoporin implicated in the Ran-GTPase cycle. And the encoded protein directly interacts with the E2 enzyme UBC9 and strongly enhances SUMO1 transfer from UBC9 to the SUMO1 target SP100. These findings place sumoylation at the cytoplasmic filaments of the nuclear pore complex and suggest that, for some substrates, modification and nuclear import are linked events. This gene is partially duplicated in a gene cluster that lies in a hot spot for recombination on chromosome 2q.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00981 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RanBP2 using anti-RanBP2 antibody (A00981).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: mouse brain tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RanBP2 antigen affinity purified polyclonal antibody (Catalog # A00981) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RanBP2 at approximately 270KD. The expected band size for RanBP2 is at 358KD.
Protein Target Info & Infographic
Gene/Protein Information For RANBP2 (Source: Uniprot.org, NCBI)
Gene Name
RANBP2
Full Name
E3 SUMO-protein ligase RanBP2
Weight
358199 MW
Superfamily
RanBP2 E3 ligase family
Alternative Names
E3 SUMO-protein ligase RanBP2;6.3.2.-;358 kDa nucleoporin;Nuclear pore complex protein Nup358;Nucleoporin Nup358;Ran-binding protein 2;RanBP2;p270;RANBP2;NUP358; RANBP2 ADANE, ANE1, IIAE3, NUP358, TRP1, TRP2 RAN binding protein 2 E3 SUMO-protein ligase RanBP2|358 kDa nucleoporin|E3 SUMO-protein transferase RanBP2|P270|acute necrotizing encephalopathy 1 (autosomal dominant)|nuclear pore complex protein Nup358|nucleoporin 358|nucleoporin Nup358|ran-binding protein 2|transformation-related protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RANBP2, check out the RANBP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RANBP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RanBP2 Antibody Picoband® (A00981)
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Customer Q&As
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5 Customer Q&As for Anti-RanBP2 Antibody Picoband®
Question
Do you have a BSA free version of anti-RanBP2 antibody A00981 available?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-RanBP2 antibody A00981 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-16
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-RanBP2 antibody A00981. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-27
Question
Would anti-RanBP2 antibody A00981 work on canine WB with biceps brachii?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
Our lab technicians have not validated anti-RanBP2 antibody A00981 on canine. You can run a BLAST between canine and the immunogen sequence of anti-RanBP2 antibody A00981 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine biceps brachii in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-20
Question
I was wanting to use your anti-RanBP2 antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
As indicated on the product datasheet, A00981 anti-RanBP2 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-13
Question
We are currently using anti-RanBP2 antibody A00981 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
F. Kulkarni
Verified customer
Asked: 2015-10-14
Answer
The anti-RanBP2 antibody (A00981) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-10-14
