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Product Info Summary
| SKU: | A00721-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-OTC Antibody (Center)
View all Ornithine Carbamoyltransferase Antibodies
SKU/Catalog Number
A00721-2
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-OTC Antibody (Center) (Catalog # A00721-2). Tested in IHC-P, Flow Cytometry, WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-OTC Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00721-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This OTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-98 amino acids of human OTC.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00721-2 is reactive to OTC in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
39935 Da
Background of Ornithine Carbamoyltransferase
OTC is a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00721-2 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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All lanes : Anti-OTC Antibody (Center) at 1:2000 dilution
Lane 1: Human liver lysate
Lane 2: Mouse liver lysate
Lane 3: Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 40 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Formalin-fixed and paraffin-embedded human hepatoarcinoma with OTC Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of ATDC5 cells using OTC Antibody (Center)(bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For OTC (Source: Uniprot.org, NCBI)
Gene Name
OTC
Full Name
Ornithine carbamoyltransferase, mitochondrial
Weight
39935 Da
Superfamily
aspartate/ornithine carbamoyltransferase superfamily
Alternative Names
Ornithine carbamoyltransferase, mitochondrial, Ornithine transcarbamylase, OTCase, OTC OTC OCTDD, OTC ornithine transcarbamylase ornithine transcarbamylase, mitochondrial|OTCase|ornithine carbamoyltransferase, mitochondrial
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OTC, check out the OTC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OTC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OTC Antibody (Center) (A00721-2)
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