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Product Info Summary
| SKU: | A01291-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human Cannabinoid Receptor I DyLight® 550 conjugated CNR1 Antibody
View all Cannabinoid R1/CB1/CNR1 Antibodies
SKU/Catalog Number
A01291-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human Cannabinoid Receptor I DyLight® 550 conjugated CNR1 Antibody catalog # A01291-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human Cannabinoid Receptor I DyLight® 550 conjugated CNR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01291-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Cannabinoid Receptor I recombinant protein (Position: M1-Q75).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01291-Dyl550 is reactive to CNR1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
52.858kDa
Background of Cannabinoid R1/CB1/CNR1
The cannabinoid receptor type 1, often abbreviated as CB1, is a G protein-coupled cannabinoid receptor located primarily in the central and peripheral nervous system. This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01291-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For CNR1 (Source: Uniprot.org, NCBI)
Gene Name
CNR1
Full Name
Cannabinoid receptor 1
Weight
52.858kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Cannabinoid receptor 1; CB-R; CB1; CANN6; CNR1; CNR CNR1 CANN6, CB-R, CB1, CB1A, CB1K5, CB1R, CNR cannabinoid receptor 1 cannabinoid receptor 1|cannabinoid receptor 1 (brain)|central cannabinoid receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CNR1, check out the CNR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CNR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human Cannabinoid Receptor I DyLight® 550 conjugated CNR1 Antibody (A01291-Dyl550)
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Human Cannabinoid Receptor I DyLight® 550 conjugated CNR1 Antibody
Question
I was wanting to use your anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody for Flow Cytometry for human lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung identification?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
You can see on the product datasheet, A01291-Dyl550 anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody A01291-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-22
Question
you antibody to test anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody A01291-Dyl550 on human lung for research purposes, then I may be interested in using anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody A01291-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
The products we sell, including anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody A01291-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-31
Question
We are currently using anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody A01291-Dyl550 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
T. Jackson
Verified customer
Asked: 2019-11-08
Answer
The anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody (A01291-Dyl550) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-08
Question
Here is the WB image, lot number and protocol we used for lung using anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody A01291-Dyl550. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-22
Question
We have observed staining in human lung. Are there any suggestions? Is anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody supposed to stain lung positively?
J. Walker
Verified customer
Asked: 2019-10-17
Answer
Based on literature lung does express CNR1. Based on Uniprot.org, CNR1 is expressed in caudate nucleus, brain stem, lung, fetal brain, hippocampus, brain tumor, brain, among other tissues. Regarding which tissues have CNR1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 17895407
Brain stem, Pubmed ID: 1718258, 2263478
Fetal brain, Pubmed ID: 15620723
Hippocampus, Pubmed ID: 14702039
Lung, Pubmed ID: 7876112, 15489334
Boster Scientific Support
Answered: 2019-10-17
Question
Is a blocking peptide available for product anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody (A01291-Dyl550)?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
We do provide the blocking peptide for product anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody (A01291-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-01
Question
My boss were satisfied with the WB result of your anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody. However we have observed positive staining in lung cell membrane using this antibody. Is that expected? Could you tell me where is CNR1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
From literature, lung does express CNR1. Generally CNR1 expresses in cell membrane. Regarding which tissues have CNR1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 17895407
Brain stem, Pubmed ID: 1718258, 2263478
Fetal brain, Pubmed ID: 15620723
Hippocampus, Pubmed ID: 14702039
Lung, Pubmed ID: 7876112, 15489334
Boster Scientific Support
Answered: 2019-10-01
Question
I see that the anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody A01291-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-12-11
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-12-11
Question
Do you have a BSA free version of anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody A01291-Dyl550 available?
Verified Customer
Verified customer
Asked: 2018-04-18
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody A01291-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-18
Question
Will A01291-Dyl550 anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-18
Answer
It shows on the product datasheet, A01291-Dyl550 anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-18
Question
Does anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody A01291-Dyl550 work for Flow Cytometry with lung?
O. Miller
Verified customer
Asked: 2016-12-08
Answer
According to the expression profile of lung, CNR1 is highly expressed in lung. So, it is likely that anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody A01291-Dyl550 will work for Flow Cytometry with lung.
Boster Scientific Support
Answered: 2016-12-08
Question
Is this A01291-Dyl550 anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody reactive to the isotypes of CNR1?
F. Williams
Verified customer
Asked: 2013-07-25
Answer
The immunogen of A01291-Dyl550 anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody is E. coli-derived human Cannabinoid Receptor I recombinant protein (Position: M1-Q75). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-07-25
Question
Can you help my question with product A01291-Dyl550, anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Mangal
Verified customer
Asked: 2013-02-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01291-Dyl550 anti-Human Cannabinoid Receptor I DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-02-13
