Product Info Summary
SKU: | A02174-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Rad23B Antibody
SKU/Catalog Number
A02174-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Rad23B Antibody catalog # A02174-2. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rad23B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02174-2)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Rad23B
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02174-2 is reactive to RAD23B in Human, Mouse, Rat
Applications
A02174-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
43171 MW
Background of hHR23b
Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity. Deconjugates SUMO1 from CLOCK, which decreases its transcriptional activation activity.
Taihei Yamaguchi, Mol. Cell. Biol., Jun 2005; 25: 5171 - 5182.
Jinke Cheng, Mol. Cell. Biol., Jul 2004; 24: 6021 - 6028.
Molly A. Brewer, AACR Meeting Abstracts, Mar 2004; 2004: 1006.
Imke M. Veltman, Hum. Mol. Genet., Jul 2005; 14: 1955 - 1963.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
![a02174 2 anti rad23b antibody ihc testing 2 a02174 2 anti rad23b antibody ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a02174-2-anti-rad23b-antibody-ihc-testing-2.jpg)
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Figure 2. Immunohistochemistry validation of RAD23B using Anti-Rad23B Antibody (A02174-2).
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C![a02174 2 anti rad23b antibody ihc testing 3 a02174 2 anti rad23b antibody ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a02174-2-anti-rad23b-antibody-ihc-testing-3.jpg)
Click image to see more details
Figure 3. Immunohistochemistry validation of RAD23B using Anti-Rad23B Antibody (A02174-2).
For more protocol information of IHC
![a02174 2 anti rad23b antibody wb testing 1 a02174 2 anti rad23b antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a02174-2-anti-rad23b-antibody-wb-testing-1.jpg)
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Figure 1. Western blotting validation for Anti-Rad23B Antibody A02174-2
Western Blot (WB) analysis of specific cells using Rad23B polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For RAD23B (Source: Uniprot.org, NCBI)
Gene Name
RAD23B
Full Name
UV excision repair protein RAD23 homolog B
Weight
43171 MW
Superfamily
RAD23 family
Alternative Names
HHR23B; HR23BUV excision repair protein RAD23 homolog B; P58; RAD23 (S. cerevisiae) homolog B; RAD23 homolog B (S. cerevisiae); RAD23, yeast homolog of, B; XP-C repair complementing complex 58 kDa; XP-C repair complementing protein; XP-C repair-complementing complex 58 kDa protein RAD23B HHR23B, HR23B, P58 RAD23 homolog B, nucleotide excision repair protein UV excision repair protein RAD23 homolog B|RAD23, yeast homolog of, B|XP-C repair complementing complex 58 kDa|XP-C repair complementing protein|XP-C repair-complementing complex 58 kDa protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAD23B, check out the RAD23B Infographic
![RAD23B infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAD23B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rad23B Antibody (A02174-2)
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