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Product Info Summary
| SKU: | A09203-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-IL1RL2 Antibody (Center)
View all IL-1Rrp2/IL-1R6 Antibodies
SKU/Catalog Number
A09203-1
Size
80 µl
Description
Boster Bio Anti-IL1RL2 Antibody (Center) (Catalog # A09203-1). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-IL1RL2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09203-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This IL1RL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the Central region of human IL1RL2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09203-1 is reactive to IL1RL2 in Human
Reconstitution
Calculated molecular weight
65405 Da
Background of IL-1Rrp2/IL-1R6
The protein encoded by this gene is a member of the interleukin 1 receptor family. An experiment with transient gene expression demonstrated that this receptor was incapable of binding to interleukin 1 alpha and interleukin 1 beta with high affinity. This gene and four other interleukin 1 receptor family genes, including interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 1 (IL1RL1), and interleukin 18 receptor 1 (IL18R1), form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. [provided by RefSeq].
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09203-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:8000
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-IL1RL2 Antibody (Center) at 1:8000 dilution
Lane 1: HL-60 whole cell lysate
Lane 2: U-87 MG whole cell lysate
Lane 3: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 65 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing K562 cells stained with A09203-1 (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A09203-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For IL1RL2 (Source: Uniprot.org, NCBI)
Gene Name
IL1RL2
Full Name
Interleukin-1 receptor-like 2
Weight
65405 Da
Superfamily
interleukin-1 receptor family
Alternative Names
Interleukin-1 receptor-like 2, IL-36 receptor, IL-36R, Interleukin-1 receptor-related protein 2, IL-1Rrp2, IL1R-rp2, IL1RL2, IL1RRP2 IL1RL2 IL-1Rrp2, IL-36R, IL1R-rp2, IL1RRP2 interleukin 1 receptor like 2 interleukin-1 receptor-like 2|IL-1 receptor-related protein 2|IL-36 receptor|interleukin-1 receptor-related protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL1RL2, check out the IL1RL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL1RL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL1RL2 Antibody (Center) (A09203-1)
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