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Product Info Summary
| SKU: | M00041 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Rac1/2/3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00041
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Rac1/2/3 Rabbit Monoclonal Antibody catalog # M00041. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rac1/2/3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00041)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAIO-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Rac1/2/3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00041 is reactive to RAC1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
83, 88 kDa
Calculated molecular weight
21450 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00041 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
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Western blot analysis of Rac1/2/3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For RAC1 (Source: Uniprot.org, NCBI)
Gene Name
RAC1
Full Name
Ras-related C3 botulinum toxin substrate 1
Weight
21450 MW
Superfamily
small GTPase superfamily
Alternative Names
Ras-related C3 botulinum toxin substrate 1;Cell migration-inducing gene 5 protein;Ras-like protein TC25;p21-Rac1;RAC1;TC25;MIG5; RAC1 MIG5, MRD48, Rac-1, TC-25, p21-Rac1 Rac family small GTPase 1 ras-related C3 botulinum toxin substrate 1|cell migration-inducing gene 5 protein|ras-like protein TC25|ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAC1, check out the RAC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rac1/2/3 Rabbit Monoclonal Antibody (M00041)
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No publications found for M00041
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Customer Q&As
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16 Customer Q&As for Anti-Rac1/2/3 Rabbit Monoclonal Antibody
Question
Can M00041 be used for immunofluorescence?
Verified customer
Asked: 2021-11-12
Answer
The Anti-Rac1/2/3 Rabbit Monoclonal Antibody (M00041) is suitable for WB only. It could not be used for immunofluorescence.
Boster Scientific Support
Answered: 2021-11-14
Question
Do you have a BSA free version of anti-Rac1/2/3 Rabbit Monoclonal antibody M00041 available?
L. Carter
Verified customer
Asked: 2020-04-08
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Rac1/2/3 Rabbit Monoclonal antibody M00041 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-08
Question
We have been able to see staining in human melanoma. What should we do? Is anti-Rac1/2/3 Rabbit Monoclonal antibody supposed to stain melanoma positively?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
According to literature melanoma does express RAC1. According to Uniprot.org, RAC1 is expressed in esophagus squamous epithelium, colon skin, pancreas skin, melanoma, cervix carcinoma, among other tissues. Regarding which tissues have RAC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Colon, and Skin, Pubmed ID: 10597294
Melanoma, Pubmed ID: 17081065
Pancreas, and Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-02-18
Question
Is this M00041 anti-Rac1/2/3 Rabbit Monoclonal antibody reactive to the isotypes of RAC1?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
The immunogen of M00041 anti-Rac1/2/3 Rabbit Monoclonal antibody is A synthesized peptide derived from human Rac1/2/3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-21
Question
My question regarding product M00041, anti-Rac1/2/3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00041 anti-Rac1/2/3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-11
Question
Does anti-Rac1/2/3 Rabbit Monoclonal antibody M00041 work for WB with pancreas skin?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
According to the expression profile of pancreas skin, RAC1 is highly expressed in pancreas skin. So, it is likely that anti-Rac1/2/3 Rabbit Monoclonal antibody M00041 will work for WB with pancreas skin.
Boster Scientific Support
Answered: 2019-07-25
Question
I am interested in to test anti-Rac1/2/3 Rabbit Monoclonal antibody M00041 on human pancreas skin for research purposes, then I may be interested in using anti-Rac1/2/3 Rabbit Monoclonal antibody M00041 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Miller
Verified customer
Asked: 2019-05-16
Answer
The products we sell, including anti-Rac1/2/3 Rabbit Monoclonal antibody M00041, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-16
Question
I am interested in using your anti-Rac1/2/3 Rabbit Monoclonal antibody for rho gtpases activate wasps and waves studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
We appreciate your inquiry. This M00041 anti-Rac1/2/3 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-06
Question
I see that the anti-Rac1/2/3 Rabbit Monoclonal antibody M00041 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-09-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-03
Question
I have attached the WB image, lot number and protocol we used for pancreas skin using anti-Rac1/2/3 Rabbit Monoclonal antibody M00041. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-08-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-17
Question
I was wanting to use your anti-Rac1/2/3 Rabbit Monoclonal antibody for WB for human pancreas skin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pancreas skin identification?
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
It shows on the product datasheet, M00041 anti-Rac1/2/3 Rabbit Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human pancreas skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-13
Question
Will M00041 anti-Rac1/2/3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Jones
Verified customer
Asked: 2017-08-15
Answer
You can see on the product datasheet, M00041 anti-Rac1/2/3 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-15
Question
We are currently using anti-Rac1/2/3 Rabbit Monoclonal antibody M00041 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
J. Parker
Verified customer
Asked: 2017-04-10
Answer
The anti-Rac1/2/3 Rabbit Monoclonal antibody (M00041) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-04-10
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas skin using anti-Rac1/2/3 Rabbit Monoclonal antibody M00041. Let me know if you need anything else.
M. Brown
Verified customer
Asked: 2014-09-26
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-09-26
Question
Is a blocking peptide available for product anti-Rac1/2/3 Rabbit Monoclonal antibody (M00041)?
E. Anderson
Verified customer
Asked: 2013-12-10
Answer
We do provide the blocking peptide for product anti-Rac1/2/3 Rabbit Monoclonal antibody (M00041). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-12-10
Question
My colleagues were satisfied with the WB result of your anti-Rac1/2/3 Rabbit Monoclonal antibody. However we have seen positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is RAC1 supposed to be expressed?
L. Carter
Verified customer
Asked: 2013-02-14
Answer
From what I have seen in literature, cervix carcinoma does express RAC1. Generally RAC1 expresses in cell membrane. Regarding which tissues have RAC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Colon, and Skin, Pubmed ID: 10597294
Melanoma, Pubmed ID: 17081065
Pancreas, and Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-02-14
