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Product Info Summary
| SKU: | PB9787 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Rab5/RAB5A Antibody Picoband®
SKU/Catalog Number
PB9787
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Rab5/RAB5A Antibody Picoband® catalog # PB9787. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab5/RAB5A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9787)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Rab5, different from the related mouse and rat sequences by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9787 is reactive to RAB5A in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
24 kDa
Calculated molecular weight
23659 MW
Background of RAB5A
RAB5A (Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling. It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP). RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. In addition, RAB5 activation is either continuous or repetitive for up to 10 minutes, but it ends before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9787 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue, HELA Whole Cell, SW620 Whole Cell, 293T Whole Cell, JURKAT Whole Cell
IHC: human intestinal cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Rab5 using anti-Rab5 antibody (PB9787).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Mouse Brain Tissue Lysate at 50ug,
Lane 3: HELA Whole Cell Lysate at 40ug,
Lane 4: SW620 Whole Cell Lysate at 40ug,
Lane 5: 293T Whole Cell Lysate at 40ug,
Lane 6: JURKAT Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab5 antigen affinity purified polyclonal antibody (Catalog # PB9787) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab5 at approximately 24 kDa. The expected band size for Rab5 is at 24 kDa.
Click image to see more details
Figure 2. IHC analysis of Rab5 using anti-Rab5 antibody (PB9787).
Rab5 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Rab5 Antibody (PB9787) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For RAB5A (Source: Uniprot.org, NCBI)
Gene Name
RAB5A
Full Name
Ras-related protein Rab-5A
Weight
23659 MW
Superfamily
small GTPase superfamily
Alternative Names
Ras-related protein Rab-5A;RAB5A;RAB5; RAB5A RAB5 RAB5A, member RAS oncogene family ras-related protein Rab-5A|RAS-associated protein RAB5A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB5A, check out the RAB5A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rab5/RAB5A Antibody Picoband® (PB9787)
Hello CJ!
PB9787 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Han F, Jiang J, Ding J, Liu H, Xiao B, Shi Y. Front Behav Neurosci. 2017 Apr 26;11:62. doi: 10.3389/fnbeh.2017.00062. eCollection 2017. Change of Rin1 and Stathmin in the Animal Model of Traumatic Stresses
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Customer Q&As
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16 Customer Q&As for Anti-Rab5/RAB5A Antibody Picoband®
Question
I was wanting to use to test anti-Rab5/RAB5A antibody PB9787 on mouse brain for research purposes, then I may be interested in using anti-Rab5/RAB5A antibody PB9787 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
The products we sell, including anti-Rab5/RAB5A antibody PB9787, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-10
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Rab5/RAB5A antibody PB9787. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-08
Question
Our lab used your anti-Rab5/RAB5A antibody for WB on brain subthalamic nucleus a few years ago. I am using mouse, and We want to use the antibody for IHC next. My question regards examining brain subthalamic nucleus as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
I viewed the website and datasheets of our anti-Rab5/RAB5A antibody and it seems that PB9787 has been tested on mouse in both WB and IHC. Thus PB9787 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-11
Question
Does anti-Rab5/RAB5A antibody PB9787 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
According to the expression profile of brain, RAB5A is highly expressed in brain. So, it is likely that anti-Rab5/RAB5A antibody PB9787 will work for WB with brain.
Boster Scientific Support
Answered: 2019-06-06
Question
My question regarding product PB9787, anti-Rab5/RAB5A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9787 anti-Rab5/RAB5A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-22
Question
See attached the WB image, lot number and protocol we used for brain using anti-Rab5/RAB5A antibody PB9787. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-14
Question
We are currently using anti-Rab5/RAB5A antibody PB9787 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
The anti-Rab5/RAB5A antibody (PB9787) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-09
Question
Does PB9787 anti-Rab5/RAB5A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-11-13
Answer
You can see on the product datasheet, PB9787 anti-Rab5/RAB5A antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-13
Question
I see that the anti-Rab5/RAB5A antibody PB9787 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-10-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-23
Question
Is this PB9787 anti-Rab5/RAB5A antibody reactive to the isotypes of RAB5A?
Verified Customer
Verified customer
Asked: 2018-10-11
Answer
The immunogen of PB9787 anti-Rab5/RAB5A antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Rab5 (179-215aa KKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN), different from the related mouse and rat sequences by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-11
Question
Is a blocking peptide available for product anti-Rab5/RAB5A antibody (PB9787)?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
We do provide the blocking peptide for product anti-Rab5/RAB5A antibody (PB9787). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-07-12
Question
My boss were well pleased with the WB result of your anti-Rab5/RAB5A antibody. However we have observed positive staining in substantia nigra cell membrane using this antibody. Is that expected? Could you tell me where is RAB5A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
According to literature, substantia nigra does express RAB5A. Generally RAB5A expresses in cell membrane. Regarding which tissues have RAB5A expression, here are a few articles citing expression in various tissues:
Brain, and Subthalamic nucleus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 11062261, 18669648
Cervix, and Placenta, Pubmed ID: 15489334
Melanoma, Pubmed ID: 17081065
Boster Scientific Support
Answered: 2018-06-18
Question
I was wanting to use your anti-Rab5/RAB5A antibody for WB for mouse brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse brain identification?
C. Miller
Verified customer
Asked: 2016-07-01
Answer
You can see on the product datasheet, PB9787 anti-Rab5/RAB5A antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-07-01
Question
Do you have a BSA free version of anti-Rab5/RAB5A antibody PB9787 available?
W. Mangal
Verified customer
Asked: 2016-06-20
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Rab5/RAB5A antibody PB9787 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-06-20
Question
We have been able to see staining in rat cervix carcinoma. Any tips? Is anti-Rab5/RAB5A antibody supposed to stain cervix carcinoma positively?
N. Moore
Verified customer
Asked: 2013-08-08
Answer
According to literature cervix carcinoma does express RAB5A. According to Uniprot.org, RAB5A is expressed in substantia nigra, brain, brain subthalamic nucleus, cervix placenta, cervix carcinoma, melanoma, among other tissues. Regarding which tissues have RAB5A expression, here are a few articles citing expression in various tissues:
Brain, and Subthalamic nucleus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 11062261, 18669648
Cervix, and Placenta, Pubmed ID: 15489334
Melanoma, Pubmed ID: 17081065
Boster Scientific Support
Answered: 2013-08-08
Question
I was wanting to use using your anti-Rab5/RAB5A antibody for post-translational protein modification studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Z. Bhatt
Verified customer
Asked: 2013-04-18
Answer
We appreciate your inquiry. This PB9787 anti-Rab5/RAB5A antibody is validated on rat brain tissue, tissue lysate, mouse brain, hela whole cell lysate, sw620 whole cell lysate, 293t whole cell lysate, jurkat whole cell lysate, intestinal cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-04-18
