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Product Info Summary
| SKU: | M01891 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Rab5 RAB5A Rabbit Monoclonal Antibody
SKU/Catalog Number
M01891
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Rab5 RAB5A Rabbit Monoclonal Antibody catalog # M01891. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab5 RAB5A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01891)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EOE-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Rab5
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01891 is reactive to RAB5A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
270 kDa
Calculated molecular weight
23659 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01891 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
IHC: human colon tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of Rab5 expression in MCF-7 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human colon, using Rab5 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For RAB5A (Source: Uniprot.org, NCBI)
Gene Name
RAB5A
Full Name
Ras-related protein Rab-5A
Weight
23659 MW
Superfamily
small GTPase superfamily
Alternative Names
Ras-related protein Rab-5A;RAB5A;RAB5; RAB5A RAB5 RAB5A, member RAS oncogene family ras-related protein Rab-5A|RAS-associated protein RAB5A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB5A, check out the RAB5A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rab5 RAB5A Rabbit Monoclonal Antibody (M01891)
Hello CJ!
M01891 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Han F, Jiang J, Ding J, Liu H, Xiao B, Shi Y. Front Behav Neurosci. 2017 Apr 26;11:62. doi: 10.3389/fnbeh.2017.00062. eCollection 2017. Change of Rin1 and Stathmin in the Animal Model of Traumatic Stresses
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Customer Q&As
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16 Customer Q&As for Anti-Rab5 RAB5A Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-Rab5 Rabbit Monoclonal antibody (M01891)?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
We do provide the blocking peptide for product anti-Rab5 Rabbit Monoclonal antibody (M01891). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-17
Question
We have seen staining in human substantia nigra. Any tips? Is anti-Rab5 Rabbit Monoclonal antibody supposed to stain substantia nigra positively?
S. Mitchell
Verified customer
Asked: 2019-12-12
Answer
From literature substantia nigra does express RAB5A. From Uniprot.org, RAB5A is expressed in substantia nigra, brain, brain subthalamic nucleus, cervix placenta, cervix carcinoma, melanoma, among other tissues. Regarding which tissues have RAB5A expression, here are a few articles citing expression in various tissues:
Brain, and Subthalamic nucleus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 11062261, 18669648
Cervix, and Placenta, Pubmed ID: 15489334
Melanoma, Pubmed ID: 17081065
Boster Scientific Support
Answered: 2019-12-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for substantia nigra using anti-Rab5 Rabbit Monoclonal antibody M01891. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-06
Question
I was wanting to use your anti-Rab5 Rabbit Monoclonal antibody for WB for human substantia nigra on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human substantia nigra identification?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
You can see on the product datasheet, M01891 anti-Rab5 Rabbit Monoclonal antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human substantia nigra in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-02
Question
My team were well pleased with the WB result of your anti-Rab5 Rabbit Monoclonal antibody. However we have observed positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is RAB5A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
According to literature, brain does express RAB5A. Generally RAB5A expresses in cell membrane. Regarding which tissues have RAB5A expression, here are a few articles citing expression in various tissues:
Brain, and Subthalamic nucleus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 11062261, 18669648
Cervix, and Placenta, Pubmed ID: 15489334
Melanoma, Pubmed ID: 17081065
Boster Scientific Support
Answered: 2019-08-01
Question
Please see the WB image, lot number and protocol we used for substantia nigra using anti-Rab5 Rabbit Monoclonal antibody M01891. Please let me know if you require anything else.
N. Jha
Verified customer
Asked: 2019-06-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-14
Question
Would M01891 anti-Rab5 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-22
Answer
As indicated on the product datasheet, M01891 anti-Rab5 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-22
Question
Will anti-Rab5 Rabbit Monoclonal antibody M01891 work for WB with substantia nigra?
V. Li
Verified customer
Asked: 2018-10-18
Answer
According to the expression profile of substantia nigra, RAB5A is highly expressed in substantia nigra. So, it is likely that anti-Rab5 Rabbit Monoclonal antibody M01891 will work for WB with substantia nigra.
Boster Scientific Support
Answered: 2018-10-18
Question
I have a question about product M01891, anti-Rab5 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01891 anti-Rab5 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-06
Question
Is this M01891 anti-Rab5 Rabbit Monoclonal antibody reactive to the isotypes of RAB5A?
K. Li
Verified customer
Asked: 2018-02-02
Answer
The immunogen of M01891 anti-Rab5 Rabbit Monoclonal antibody is A synthesized peptide derived from human Rab5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-02-02
Question
We are currently using anti-Rab5 Rabbit Monoclonal antibody M01891 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
The anti-Rab5 Rabbit Monoclonal antibody (M01891) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-05
Question
I see that the anti-Rab5 Rabbit Monoclonal antibody M01891 works with WB, what is the protocol used to produce the result images on the product page?
G. Wu
Verified customer
Asked: 2017-08-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-08-15
Question
We ordered your anti-Rab5 Rabbit Monoclonal antibody for WB on melanoma in a previous project. I am using mouse, and We want to use the antibody for IHC next. Our lab want to know about examining melanoma as well as cervix carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
J. Baker
Verified customer
Asked: 2015-04-22
Answer
I have checked the website and datasheets of our anti-Rab5 Rabbit Monoclonal antibody and it appears that M01891 has been validated on mouse in both WB and IHC. Thus M01891 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-04-22
Question
Does anti-Rab5 Rabbit Monoclonal antibody M01891 work on bovine WB with cervix placenta?
V. Li
Verified customer
Asked: 2013-12-10
Answer
Our lab technicians have not validated anti-Rab5 Rabbit Monoclonal antibody M01891 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Rab5 Rabbit Monoclonal antibody M01891 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine cervix placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-12-10
Question
We want to test anti-Rab5 Rabbit Monoclonal antibody M01891 on human substantia nigra for research purposes, then I may be interested in using anti-Rab5 Rabbit Monoclonal antibody M01891 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
F. Miller
Verified customer
Asked: 2013-02-07
Answer
The products we sell, including anti-Rab5 Rabbit Monoclonal antibody M01891, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-02-07
Question
Is there a BSA free version of anti-Rab5 Rabbit Monoclonal antibody M01891 available?
B. Edwards
Verified customer
Asked: 2013-01-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Rab5 Rabbit Monoclonal antibody M01891 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-01-17
