Product Info Summary
SKU: | A04789-2 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RAB3GAP1 Antibody Picoband™
SKU/Catalog Number
A04789-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RAB3GAP1 Antibody Picoband™ catalog # A04789-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RAB3GAP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04789-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RAB3GAP1 recombinant protein (Position: N109-Q810).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04789-2 is reactive to RAB3GAP1 in Human
Applications
A04789-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
130 kDa
Calculated molecular weight
Background of RAB3GAP1
Rab3 GTPase-activating protein catalytic subunit is an enzyme that in humans is encoded by the RAB3GAP1 gene. This gene encodes the catalytic subunit of a Rab GTPase activating protein. The encoded protein forms a heterodimer with a non-catalytic subunit to specifically regulate the activity of members of the Rab3 subfamily of small G proteins. This protein mediates the hydrolysis of GTP bound Rab3 to the GDP bound form. Mutations in this gene are associated with Warburg micro syndrome. Alternate splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a04789 2 rab3gap1 primary antibodies wb testing 1 a04789 2 rab3gap1 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a04789-2-rab3gap1-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB3GAP1 antigen affinity purified polyclonal antibody (Catalog # A04789-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB3GAP1 at approximately 130 kDa. The expected band size for RAB3GAP1 is at 111 kDa.
![a04789 2 rab3gap1 primary antibodies ihc testing 2 a04789 2 rab3gap1 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a04789-2-rab3gap1-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2).
RAB3GAP1 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (A04789-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a04789 2 rab3gap1 primary antibodies ihc testing 3 a04789 2 rab3gap1 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a04789-2-rab3gap1-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2).
RAB3GAP1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (A04789-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a04789 2 rab3gap1 primary antibodies ihc testing 4 a04789 2 rab3gap1 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a04789-2-rab3gap1-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2).
RAB3GAP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (A04789-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a04789 2 rab3gap1 primary antibodies ihc testing 5 a04789 2 rab3gap1 primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a04789-2-rab3gap1-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2).
RAB3GAP1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (A04789-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a04789 2 rab3gap1 primary antibodies ihc testing 6 a04789 2 rab3gap1 primary antibodies ihc testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a04789-2-rab3gap1-primary-antibodies-ihc-testing-6.jpg)
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Figure 6. IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2).
RAB3GAP1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (A04789-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a04789 2 rab3gap1 primary antibodies ihc testing 7 a04789 2 rab3gap1 primary antibodies ihc testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a04789-2-rab3gap1-primary-antibodies-ihc-testing-7.jpg)
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Figure 7. IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2).
RAB3GAP1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (A04789-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a04789 2 rab3gap1 primary antibodies if testing 8 a04789 2 rab3gap1 primary antibodies if testing 8](https://www.bosterbio.com/media/catalog/product/a/0/a04789-2-rab3gap1-primary-antibodies-if-testing-8.jpg)
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Figure 8. IF analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2).
RAB3GAP1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RAB3GAP1 Antibody (A04789-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a04789 2 rab3gap1 primary antibodies if testing 9 a04789 2 rab3gap1 primary antibodies if testing 9](https://www.bosterbio.com/media/catalog/product/a/0/a04789-2-rab3gap1-primary-antibodies-if-testing-9.jpg)
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Figure 9. IF analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2).
RAB3GAP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RAB3GAP1 Antibody (A04789-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a04789 2 rab3gap1 primary antibodies if testing 10_2 a04789 2 rab3gap1 primary antibodies if testing 10_2](https://www.bosterbio.com/media/catalog/product/a/0/a04789-2-rab3gap1-primary-antibodies-if-testing-10_2.jpg)
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Figure 10. IF analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2) and Anti-Beta Tubulin Antibody (M01857-3).
RAB3GAP1 was detected in an immunocytochemical section of Siha cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAB3GAP1 Antibody (A04789-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a03302 2 asns primary antibodies fcm testing 11 a03302 2 asns primary antibodies fcm testing 11](https://www.bosterbio.com/media/catalog/product/a/0/a03302-2-asns-primary-antibodies-fcm-testing-11.jpg)
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Figure 11. Flow Cytometry analysis of HL-60 cells using anti-RAB3GAP1 antibody (A04789-2).
Overlay histogram showing HL-60 cells stained with A04789-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB3GAP1 Antibody (A04789-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RAB3GAP1 (Source: Uniprot.org, NCBI)
Gene Name
RAB3GAP1
Full Name
Rab3 GTPase-activating protein catalytic subunit
Weight
Superfamily
Rab3-GAP catalytic subunit family
Alternative Names
KIAA0066P130; RAB3 GTPase activating protein subunit 1 (catalytic); RAB3 GTPase-activating protein 130 kDa subunit; rab3 GTPase-activating protein catalytic subunit; Rab3-GAP p130; Rab3-GAP; RAB3GAPDKFZp434A012; WARBM1RAB3GAP130 RAB3GAP1 P130, RAB3GAP30, WARBM1, RAB3GAP1 RAB3 GTPase activating protein catalytic subunit 1 rab3 GTPase-activating protein catalytic subunit|RAB3 GTPase activating protein subunit 1 (catalytic)|RAB3 GTPase-activating protein 130 kDa subunit|rab3-GAP p130
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB3GAP1, check out the RAB3GAP1 Infographic
![RAB3GAP1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB3GAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAB3GAP1 Antibody Picoband™ (A04789-2)
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