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Product Info Summary
| SKU: | A04789 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Rab 3 GAP p130 RAB3GAP1 Antibody
SKU/Catalog Number
A04789
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Rab 3 GAP p130 RAB3GAP1 Antibody catalog # A04789. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab 3 GAP p130 RAB3GAP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04789)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RAB3GAP1. AA range:538-587
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04789 is reactive to RAB3GAP1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
110524 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04789 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using Rab 3 GAP p130 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For RAB3GAP1 (Source: Uniprot.org, NCBI)
Gene Name
RAB3GAP1
Full Name
Rab3 GTPase-activating protein catalytic subunit
Weight
110524 MW
Superfamily
Rab3-GAP catalytic subunit family
Alternative Names
Rab3 GTPase-activating protein catalytic subunit;RAB3 GTPase-activating protein 130 kDa subunit;Rab3-GAP p130;Rab3-GAP;RAB3GAP1;KIAA0066, RAB3GAP; RAB3GAP1 P130, RAB3GAP30, WARBM1, RAB3GAP1 RAB3 GTPase activating protein catalytic subunit 1 rab3 GTPase-activating protein catalytic subunit|RAB3 GTPase activating protein subunit 1 (catalytic)|RAB3 GTPase-activating protein 130 kDa subunit|rab3-GAP p130
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB3GAP1, check out the RAB3GAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB3GAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rab 3 GAP p130 RAB3GAP1 Antibody (A04789)
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No publications found for A04789
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Customer Q&As
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13 Customer Q&As for Anti-Rab 3 GAP p130 RAB3GAP1 Antibody
Question
I see that the anti-Rab 3 GAP p130 antibody A04789 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-27
Question
I have a question about product A04789, anti-Rab 3 GAP p130 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04789 anti-Rab 3 GAP p130 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-11
Question
I am looking for to test anti-Rab 3 GAP p130 antibody A04789 on human brodmann (1909) area 23 for research purposes, then I may be interested in using anti-Rab 3 GAP p130 antibody A04789 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
The products we sell, including anti-Rab 3 GAP p130 antibody A04789, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-03
Question
Here is the WB image, lot number and protocol we used for brodmann (1909) area 23 using anti-Rab 3 GAP p130 antibody A04789. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-13
Question
We are currently using anti-Rab 3 GAP p130 antibody A04789 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
The anti-Rab 3 GAP p130 antibody (A04789) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-23
Question
Would A04789 anti-Rab 3 GAP p130 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-06-27
Answer
You can see on the product datasheet, A04789 anti-Rab 3 GAP p130 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-27
Question
Would anti-Rab 3 GAP p130 antibody A04789 work for WB with brodmann (1909) area 23?
B. Parker
Verified customer
Asked: 2017-10-31
Answer
According to the expression profile of brodmann (1909) area 23, RAB3GAP1 is highly expressed in brodmann (1909) area 23. So, it is likely that anti-Rab 3 GAP p130 antibody A04789 will work for WB with brodmann (1909) area 23.
Boster Scientific Support
Answered: 2017-10-31
Question
I was wanting to use your anti-Rab 3 GAP p130 antibody for WB for human brodmann (1909) area 23 on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brodmann (1909) area 23 identification?
Z. Walker
Verified customer
Asked: 2017-04-06
Answer
As indicated on the product datasheet, A04789 anti-Rab 3 GAP p130 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brodmann (1909) area 23 in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-06
Question
Would anti-Rab 3 GAP p130 antibody A04789 work on canine WB with testis?
A. Taylor
Verified customer
Asked: 2016-12-02
Answer
Our lab technicians have not tested anti-Rab 3 GAP p130 antibody A04789 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Rab 3 GAP p130 antibody A04789 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-12-02
Question
Is this A04789 anti-Rab 3 GAP p130 antibody reactive to the isotypes of RAB3GAP1?
D. Moore
Verified customer
Asked: 2016-08-19
Answer
The immunogen of A04789 anti-Rab 3 GAP p130 antibody is Synthesized peptide derived from the Internal region of human Rab 3 GAP p130. at AA rangle: 510-590. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-08-19
Question
Is a blocking peptide available for product anti-Rab 3 GAP p130 antibody (A04789)?
M. Li
Verified customer
Asked: 2015-09-17
Answer
We do provide the blocking peptide for product anti-Rab 3 GAP p130 antibody (A04789). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-09-17
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brodmann (1909) area 23 using anti-Rab 3 GAP p130 antibody A04789. Let me know if you need anything else.
S. Li
Verified customer
Asked: 2014-10-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-10
Question
Do you have a BSA free version of anti-Rab 3 GAP p130 antibody A04789 available?
D. Collins
Verified customer
Asked: 2013-05-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Rab 3 GAP p130 antibody A04789 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-05-22
