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Product Info Summary
| SKU: | A03845-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-RAB35 Antibody (C-term)
SKU/Catalog Number
A03845-1
Size
80 µl
Description
Boster Bio Anti-RAB35 Antibody (C-term) (Catalog # A03845-1). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RAB35 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03845-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This RAB35 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 166-199 amino acids from the C-terminal region of human RAB35.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03845-1 is reactive to RAB35 in Human, Mouse, Rat
Applications
A03845-1 is guaranteed for IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
23025 Da
Background of RAB35
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysates from Hela, U-87 MG, rat PC-12 cell line , huamn brain and mouse brain tissue lysate (from left to right), using RAB35 Antibody (C-term). A03845-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Immunohistochemical analysis of paraffin-embedded H. small intestine section using RAB35 Antibody (C-term) (Cat#A03845-1). A03845-1 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Protein Target Info & Infographic
Gene/Protein Information For RAB35 (Source: Uniprot.org, NCBI)
Gene Name
RAB35
Full Name
Ras-related protein Rab-35
Weight
23025 Da
Superfamily
small GTPase superfamily
Alternative Names
GTP-binding protein RAY; H-ray; RAB1C; RAB35, member RAS oncogene family; ras-related protein rab-1c (GTP-binding protein ray); Ras-related protein Rab-1C; ras-related protein Rab-35; RAY RAB35 H-ray, RAB1C, RAY RAB35, member RAS oncogene family ras-related protein Rab-35|GTP-binding protein RAY|ras-related protein rab-1c (GTP-binding protein ray)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB35, check out the RAB35 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB35: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAB35 Antibody (C-term) (A03845-1)
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