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Product Info Summary
| SKU: | A03845 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Rab 35 Antibody
SKU/Catalog Number
A03845
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Rab 35 Antibody catalog # A03845. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab 35 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03845)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RAB35. AA range:99-148
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03845 is reactive to RAB35 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
23025 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03845 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using Rab 35 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For RAB35 (Source: Uniprot.org, NCBI)
Gene Name
RAB35
Full Name
Ras-related protein Rab-35
Weight
23025 MW
Superfamily
small GTPase superfamily
Alternative Names
Ras-related protein Rab-35;GTP-binding protein RAY;Ras-related protein Rab-1C;RAB35;RAB1C, RAY; RAB35 H-ray, RAB1C, RAY RAB35, member RAS oncogene family ras-related protein Rab-35|GTP-binding protein RAY|ras-related protein rab-1c (GTP-binding protein ray)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB35, check out the RAB35 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB35: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rab 35 Antibody (A03845)
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Customer Q&As
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4 Customer Q&As for Anti-Rab 35 Antibody
Question
We are currently using anti-Rab 35 antibody A03845 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The anti-Rab 35 antibody (A03845) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-15
Question
Will A03845 anti-Rab 35 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-03-11
Answer
As indicated on the product datasheet, A03845 anti-Rab 35 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-11
Question
Does anti-Rab 35 antibody A03845 work for WB with frontal cortex?
Verified Customer
Verified customer
Asked: 2018-08-03
Answer
According to the expression profile of frontal cortex, RAB35 is highly expressed in frontal cortex. So, it is likely that anti-Rab 35 antibody A03845 will work for WB with frontal cortex.
Boster Scientific Support
Answered: 2018-08-03
Question
Do you have a BSA free version of anti-Rab 35 antibody A03845 available?
J. Kulkarni
Verified customer
Asked: 2018-01-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Rab 35 antibody A03845 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-12
