Anti-RAB14 Antibody Picoband™

Product Name

Anti-RAB14 Antibody Picoband™

View all RAB14 Antibodies

SKU/Catalog Number

A04231-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-RAB14 Antibody Picoband™ catalog # A04231-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-RAB14 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04231-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E.coli-derived human RAB14 recombinant protein (Position: D108-C215).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A04231-1 is reactive to RAB14 in Human, Mouse, Rat

Applications

A04231-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

24 kDa

Calculated molecular weight

52588 MW

Background of RAB14

Ras-related protein Rab-14 is a protein that in humans is encoded by the RAB14 gene. It is mapped to 9q33.2 based on an alignment of the RAB14 sequence with the genomic sequence. RAB14 belongs to the large RAB family of low molecular mass GTPases that are involved in intracellular membrane trafficking. These proteins act as molecular switches that flip between an inactive GDP-bound state and an active GTP-bound state in which they recruit downstream effector proteins onto membranes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of RAB14 using anti-RAB14 antibody (A04231-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human T-47D whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human A431 whole cell lysates,
Lane 6: human U937 whole cell lysates,
Lane 7: human MCF-7 whole cell lysates,
Lane 8: rat brain tissue lysates,
Lane 9: mouse brain tissue lysates,
Lane 10: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB14 antigen affinity purified polyclonal antibody (Catalog # A04231-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB14 at approximately 24 kDa. The expected band size for RAB14 is at 24 kDa.

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Figure 2. Flow Cytometry analysis of Hela cells using anti-RAB14 antibody (A04231-1).
Overlay histogram showing Hela cells stained with A04231-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB14 Antibody (A04231-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RAB14 (Source: Uniprot.org, NCBI)

Alternative Names

bA165P4.3 (member RAS oncogene family); F protein-binding protein 1; FBP; RAB-14; RAB14, member RAS oncogene family; ras-related protein Rab-14; small GTP binding protein RAB14 RAB14 FBP, RAB-14 RAB14, member RAS oncogene family ras-related protein Rab-14|F protein-binding protein 1|bA165P4.3 (member RAS oncogene family)|small GTP binding protein RAB14

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In this infographic, you will see the following information for RAB14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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