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Product Info Summary
| SKU: | PB9789 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Rab11A Antibody Picoband®
SKU/Catalog Number
PB9789
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Rab11A Antibody Picoband® catalog # PB9789. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab11A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9789)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Rab11A, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9789 is reactive to RAB11A in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
24 kDa
Calculated molecular weight
24394 MW
Background of RAB11A
Ras-related protein Rab-11A is a protein that in humans is encoded by the RAB11A gene. The protein encoded by this gene belongs to the small GTPase superfamily, Rab family which plays essential roles in vesicle and granule targeting. It is mapped to 15q22.31. RAB11A is associated with both constitutive and regulated secretory pathways, and may be involved in protein transport. Additionally, RAB11A can control intracellular trafficking of the innate immune receptor TLR4, and thereby also receptor signaling. It has been shown to interact with RAB11FIP2, RAB11FIP4, and RAB11FIP1 and so on.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9789 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human Hela whole cell, human placenta tissue, human COLO-320 whole cell, human HepG2 whole cell, human 22RV1 whole cell, human SGC-7901 whole cell, human MDA-MB-453 whole cell, human Jurkat whole cell, rat kidney tissue, rat testis tissue, rat brain tissue, mouse testis tissue, mouse brain tissue, mouse NIH/3T3 whole cell
ICC/IF: human U20S cell
FCM: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Rab11A using anti-Rab11A antibody (PB9789).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human COLO-320 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human 22RV1 whole cell lysates,
Lane 6: human SGC-7901 whole cell lysates,
Lane 7: human MDA-MB-453 whole cell lysates,
Lane 8: human Jurkat whole cell lysates,
Lane 9: rat kidney tissue lysates,
Lane 10: rat testis tissue lysates,
Lane 11: rat brain tissue lysates,
Lane 12: mouse testis tissue lysates,
Lane 13: mouse brain tissue lysates,
Lane 14: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab11A antigen affinity purified polyclonal antibody (Catalog # PB9789) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab11A at approximately 24 kDa. The expected band size for Rab11A is at 24 kDa.
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Figure 2. IF analysis of Rab11A using anti-Rab11A antibody (PB9789)
Rab11A was detected in immunocytochemical section of human U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-Rab11A Antibody (PB9789) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of A549 cells using anti-Rab11A antibody (PB9789).
Overlay histogram showing A549 cells stained with PB9789 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rab11A Antibody (PB9789,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For RAB11A (Source: Uniprot.org, NCBI)
Gene Name
RAB11A
Full Name
Ras-related protein Rab-11A
Weight
24394 MW
Superfamily
small GTPase superfamily
Alternative Names
Ras-related protein Rab-11A;Rab-11;YL8;RAB11A;RAB11; RAB11A YL8 RAB11A, member RAS oncogene family ras-related protein Rab-11A|RAB 11A, member oncogene family|rab-11
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB11A, check out the RAB11A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB11A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rab11A Antibody Picoband® (PB9789)
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Customer Q&As
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1 Customer Q&As for Anti-Rab11A Antibody Picoband®
Question
We are currently using anti-Rab11A antibody PB9789 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
O. Gonzalez
Verified customer
Asked: 2016-12-08
Answer
The anti-Rab11A antibody (PB9789) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-12-08
