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Product Info Summary
| SKU: | M06224 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-PURB Antibody (C-term)
SKU/Catalog Number
M06224
Size
50 µl
Description
Boster Bio Anti-PURB Antibody (C-term) (Catalog # M06224). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PURB Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06224)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2117CT1176.48.6
Isotype
IgG1,κ
Immunogen
This PURB antibody is generated from a mouse immunized with a recombiant protein of human PURB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06224 is reactive to PURB in Human, Mouse
Reconstitution
Calculated molecular weight
33241 Da
Background of PURB
Has capacity to bind repeated elements in single- stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine- rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06224 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:8000
Validation Images & Assay Conditions
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All lanes : Anti-PURB Antibody (C-term) at 1:8000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: HeLa whole cell lysate
Lane 3: NCI-H460 whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: MCF-7 whole cell lysate
Lane 6: NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 33 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For PURB (Source: Uniprot.org, NCBI)
Gene Name
PURB
Full Name
Transcriptional activator protein Pur-beta
Weight
33241 Da
Superfamily
PUR DNA-binding protein family
Alternative Names
Transcriptional activator protein Pur-beta, Purine-rich element-binding protein B, PURB PURB PURBETA purine rich element binding protein B transcriptional activator protein Pur-beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PURB, check out the PURB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PURB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PURB Antibody (C-term) (M06224)
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