Product Info Summary
SKU: | M05020-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PTBP2 Picoband® Antibody (monoclonal, 13D11)
SKU/Catalog Number
M05020-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PTBP2 Picoband® Antibody (monoclonal, 13D11) catalog # M05020-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PTBP2 Picoband® Antibody (monoclonal, 13D11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05020-2)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Monoclonal
Clone Number
13D11
Isotype
Mouse IgG2a
Standard Protein
E.coli-derived human PTBP2 recombinant protein (Position: M1-A504).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M05020-2 is reactive to Ptbp2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
57-60 kDa
Background of Ptbp2
Polypyrimidine tract binding protein 2, also known as PTBP2, is a protein which in humans is encoded by the PTBP2 gene. It is mapped to 1p21.3. The protein encoded by this gene binds to intronic polypyrimidine clusters in pre-mRNA molecules and is implicated in controlling the assembly of other splicing-regulatory proteins. This protein is very similar to the polypyrimidine tract binding protein (PTB) but most of its isoforms are expressed primarily in the brain. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05020-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human SH-SY5Y whole cell, rat testis tissue, mouse testis tissue
IHC: human gallbladder adenocarcinoma tissue, mouse testis tissue, rat testis tissue
ICC/IF: U20S cell
FCM: THP-1 cell
Validation Images & Assay Conditions
![m05020 2 ptbp2 primary antibodies wb testing 1 m05020 2 ptbp2 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m05020-2-ptbp2-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of PTBP2 using anti-PTBP2 antibody (M05020-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: rat testis tissue lysates,
Lane 3: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PTBP2 antigen affinity purified monoclonal antibody (Catalog # M05020-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PTBP2 at approximately 57-60 kDa. The expected band size for PTBP2 is at 57 kDa.
![m05020 2 ptbp2 primary antibodies ihc testing 2 m05020 2 ptbp2 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m05020-2-ptbp2-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of PTBP2 using anti-PTBP2 antibody (M05020-2).
PTBP2 was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PTBP2 Antibody (M05020-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
![m05020 2 ptbp2 primary antibodies ihc testing 3 m05020 2 ptbp2 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m05020-2-ptbp2-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of PTBP2 using anti-PTBP2 antibody (M05020-2).
PTBP2 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PTBP2 Antibody (M05020-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
![m05020 2 ptbp2 primary antibodies ihc testing 4 m05020 2 ptbp2 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/m/0/m05020-2-ptbp2-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of PTBP2 using anti-PTBP2 antibody (M05020-2).
PTBP2 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PTBP2 Antibody (M05020-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
![m05020 2 ptbp2 primary antibodies if testing 5 m05020 2 ptbp2 primary antibodies if testing 5](https://www.bosterbio.com/media/catalog/product/m/0/m05020-2-ptbp2-primary-antibodies-if-testing-5.jpg)
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Figure 5. IF analysis of PTBP2 using anti-PTBP2 antibody (M05020-2).
PTBP2 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-PTBP2 Antibody (M05020-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![m05020 2 ptbp2 primary antibodies fcm testing 6 m05020 2 ptbp2 primary antibodies fcm testing 6](https://www.bosterbio.com/media/catalog/product/m/0/m05020-2-ptbp2-primary-antibodies-fcm-testing-6.jpg)
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Figure 6. Flow Cytometry analysis of THP-1 cells using anti-PTBP2 antibody (M05020-2).
Overlay histogram showing THP-1 cells stained with M05020-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PTBP2 Antibody (M05020-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Ptbp2 (Source: Uniprot.org, NCBI)
Gene Name
Ptbp2
Full Name
Polypyrimidine tract-binding protein 2
Weight
Alternative Names
brPTB; FLJ34897; neural polypyrimidine tract binding protein; Neural polypyrimidine tract-binding protein; Neurally-enriched homolog of PTB; nPTB; nPTB6; nPTB7; nPTB8; polypyrimidine tract binding protein 2; polypyrimidine tract-binding protein 2; PTB-like protein; PTBPTBLPnPTB5; splicing regulator Ptbp2|Ptb2, brP, brPTB, nPTB|polypyrimidine tract binding protein 2|polypyrimidine tract-binding protein 2|RRM-type RNA-binding protein brPTB|brain-enriched PTB|brain-enriched polypyrimidine tract-binding protein|neural polypyrimidine tract-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ptbp2, check out the Ptbp2 Infographic
![Ptbp2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ptbp2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PTBP2 Picoband® Antibody (monoclonal, 13D11) (M05020-2)
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