Ptbp2 Antibodies

Polypyrimidine tract-binding protein 2 (Ptbp2)

RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the capacity of NOVA1 to activate exon selection.

In addition to its function in pre-mRNA splicing, plays also a role in the regulation of translation. .

Gene Information

Mouse
Gene Name:	Ptbp2
Uniprot:	Q91Z31
Entrez:	56195

Family

Belongs to:
No superfamily

Alternative Names

brPTB; FLJ34897; neural polypyrimidine tract binding protein; Neural polypyrimidine tract-binding protein; Neurally-enriched homolog of PTB; nPTB; nPTB6; nPTB7; nPTB8; polypyrimidine tract binding protein 2; polypyrimidine tract-binding protein 2; PTB-like protein; PTBPTBLPnPTB5; splicing regulator

Molecular Weight

Mass (kDA):
57.489 kDA

Genomic Context

Mouse
Location:	3|3 G1
Sequence:	3;

Tissue Specificity

Mainly expressed in brain, including cerebellum, brainstem, spinal cord, and hypothalamus. Also expressed in the peripheral nervous system and neural crest derivatives, including the dorsal root and trigeminal ganglia, the cochlear spiral and intestinal ganglion cells, and the adrenal medulla. Also detected to a lower extent in testis, heart, liver, lung, skeletal muscle and thymus (at protein level).

Diseases

• Neuroblastoma
• Nervousness
• Glioma
• Obesity
• Timothy Syndrome
• Hereditary Diseases
• Long Qt Syndrome
• Neurodegenerative Disorders
• Nerve Degeneration
• Tdp-43 Proteinopathies
• Tissue Adhesions
• Diabetes Mellitus
• Progressive Supranuclear Palsy

• Diabetes Mellitus, Non-insulin-dependent
• Supranuclear Palsy
• Malignant Neoplasms
• Syndactyly
• Tauopathies
• Alzheimer's Disease
• Movement Disorders

Pathways

• Brain Development
• Neurogenesis
• Cell Differentiation
• Synapse Maturation
• Transport
• Cell Proliferation
• Cell Migration
• Stem Cell Differentiation
• Muscle Contraction
• Localization
• Rna Splicing

• Drug Resistance
• Translation
• Cell Death
• Heart Development
• Rna Interference

PTMs

• Biotinylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Ptbp2

Boster Bio - The Best Uses Of The PTBP2 Marker

In this article, we'll cover the definition of PTBP2 and its downstream targets, how to use antibodies produced by Boster Bio, and the various types of PTBP2 antibodies. We'll also look at the benefits of enhanced chemiluminescence, one of Boster's newest products. This article will provide you with the basics of PTBP2, as well as the best uses of PTBP2 in research.

PTBP2

The PTBP2 gene is one of the most commonly used markers in the immunotherapy field. It is a key transcription factor in the immune system and is expressed in multiple tissues and organs. Moreover, the gene is expressed in the brain, spinal cord, and other organs. Although its functions are numerous, PTBP1 plays a crucial role in gliomagenesis.

PTBP1 is overexpressed in glioma tumors, which may indicate that the regulatory pathway that controls the gene is dysfunctional. In addition, PTBP2 promotes cell proliferation, and deletion of its homolog in Drosophila melangaster is embryonic lethal. Interestingly, downregulation of PTBP1 results in the debilitation of normal human fibroblast cells.

PTBP1 and PTBP2 are RNA binding proteins with similar expression patterns in different tissues. While the two proteins have distinct functions in the brain, they are both expressed in high levels in the foetal brain. Furthermore, their expression levels differ, and their differential levels may influence cell fate determination. If you are interested in identifying tumor cells with PTBP1, you can buy the PTBP2 gene from Boster Bio.

PTBP1 and PTBP2 share a common target in regulating the splicing of the RTN4 gene. They both target RTN4 exon 3, which is the third exon. However, it has been shown that PTBP1 knockdown affects the ability of tumour cells to grow. Hence, knocking down PTBP1 and PTBP2 genes together would result in tumours of higher severity.

Although PTBP2 and RTN4 are two of the most important oncogenes in glioma cells, there are other mechanisms involved in this process. PTBP1 regulates RTN4 splicing, which may affect cell proliferation. By inhibiting RTN4 splicing, PTBP1 is able to regulate the RTN4 gene and increase cell motility. These mechanisms are key in glioma and cancer cell proliferation.

PTBP2 downstream targets

PTBP2 regulates RNA, but its target genes are unclear. This is where Boster comes in. The platform features gene infographics, highlighting basic information about each gene. It covers all human and mouse genes, and features a gene search bar for specific queries. Here are some examples of gene infographics. These include: (a) ptbp2; (b) sirtuin 1; and [c] snRt, a small RNA regulatory protein.

PTBP2 has multiple targets, including several splicing factors. It affects mRNA processing and alternative splicing, resulting in derepression of multiple mRNA targets. PTBP2 is thought to influence these processes through its non-canonical binding to 5'UTRs. Several factors have been validated as direct miR-10b targets in GSC.

The NOVA2 family of RNA-binding proteins is highly homologous and regulates over 700 alternative splicing events in vivo. This protein also regulates axons, the long strings of neurons that connect the left and right sides of the brain. It is not clear whether this gene affects the development of the nervous system in humans, but it is a fascinating discovery.

Boster's antibodies

PTBP1 and PTBP2 are RNA-binding proteins that affect the invasive growth properties of glioma cells. Both proteins inhibit exon splicing, resulting in increased translation of their targets. PTBP1 is also involved in the regulation of focal adhesion-encoding transcripts at the cell membrane. Moreover, these proteins may be involved in cell proliferation and invasion, but their precise roles are not known.

Both PTBP1 and PTBP2 bind to RNA regulatory sequence motifs that occur frequently in brain-specific alternative exons. Although they share many targets, they have distinct functions in gene expression, and their differential expression may influence cell fate determination. The PTBP1 and PTBP2 proteins also have overlapping but nonoverlapping RNA-binding capabilities. This suggests that their expression level may play an important role in predicting the fate of a cancer cell.

In vitro studies, PTBP2 was required for neonatal survival. This is consistent with the role of PTBP2 in myelination and axonogenesis. Interestingly, PTBP2 is expressed precociously in adult mice. Thus, the role of PTBP2 in the nervous system of neonates has been identified. As such, this discovery will help scientists understand how PTBP2 affects the development of brain cells.

The expression of PTBP2 in developing neurons is affected by the presence or absence of dynamin1 and Magi1 proteins. Both Ptbp2 is expressed in both skeletal muscle and cardiac muscle, and its absence may play a role in the death and paralysis of germline mutant mice. Thus, both the PTBP2 marker and dynamin1 are critical for the development of mature synaptically active neurons.

Enhanced chemiluminescence

PTBP2 is a protein expressed in all mammalian cells. Immunoblot analysis was performed using an enhanced chemiluminescence system (ECLS) provided by Fujifilm and Millipore. Primary antibodies used were polyclonal anti-RBM4a, anti-GAPDH, and anti-phosphorylated JNK from MDBio and R&D Systems. Monoclonal anti-SRSF3 from Abnova was used as a loading control.

Enhanced chemiluminescence uses a luminol-based detection method to detect minute amounts of biological materials. This technique is suitable for microplate-based immunoassays and DNA probe assays. The fluorescent signal is generated rapidly and fades exponentially with time, making it a very sensitive method to detect minute amounts of biological materials. The sensitivity of the technique is extremely high, and it can detect small quantities of proteins.

The increased expression of PTBP1 in human breast cancers has been associated with aggressive behavior of the tumor. This marker is also associated with the presence of her-2 in breast cancer cells. In addition, increased expression of PTBP1 in tumors is associated with higher expression of her-2 and lymph node metastasis. This means that it could be a potential target for the treatment of breast cancer.

PTBP1 was found to regulate a number of biological processes, including proliferation of breast cancer cells. It also positively regulates the PTEN/Akt signal pathway and the transition of LC3BI to LC3BII during autophagy. Hence, knockdown of this gene inhibits the growth of breast cancer cells. It also reduces the growth of cells in the subcutaneous region of nude mice.

PTBP2 is also known as NPTB or PTB and is an important regulator of RNA splicing. It is expressed in meiotic and post-meiotic germ cells. It is required for the proper alternative mRNA splicing of more than 200 genes, including a subset of genes that are involved in protein trafficking via transport ves. Further, it has been reported that PTBP2 is required for the growth of mammalian spermatogenesis.

PTBP1 mediates exon 10 removal from PTBP2 pre-mRNA

The PTBP2 gene is found in various tissues and is characterized by two types of exons, microexons 8A and 16A. The first type is called wild-type, while the second type is called variant 8. Microexon 34' incorporates about half of the mRNAs, while microexon 8A and 16A incorporate the remaining one-third of the mRNA. Microexon 34' is the most highly expressed minigene, with the remaining 50% expressing the wild-type gene.

The PTBP2 gene is also known as polypyrimidine tract binding protein. It is highly expressed in the brain. Multiple transcript variants are formed due to alternative splicing, which results in various forms. Molecular biomarkers containing this gene are a good tool for cancer research and development. Using this gene as a biomarker may improve the accuracy of a tumor marker.

The PTBP2 gene was originally identified as an oncogenic gene in non-small cell lung cancer. Its anti-oncogenic effect was subsequently shown in pancreatic ductal adenocarcinoma. Although it has not yet been confirmed, this gene may play an important role in regulating the progression of cancer through epithelial-mesenchymal transition.

Monoclonal antibodies for the PTBP2 gene are available from Boster Bio. These antibodies have high affinity and have been extensively validated in immunohistochemistry, Western blotting, and ELISA. They are also highly compatible with other antibodies, ensuring the best use for this marker. It is also possible to conjugate monoclonal antibodies for various applications. And as a bonus, Boster also offers high-affinity antibodies that can detect PTBP2 protein in human tissues.

Researchers have identified that PTBP2 gene expression is strongly associated with TNM stage, lymphatic metastasis, and distant metastasis in CRC. In addition, YAP1 expression is associated with overall survival among CRC patients. However, the role of YAP1 in tumorigenesis and angiogenesis has yet to be understood. This gene is upregulated in the colon cells of patients suffering from colorectal cancer.