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- Table of Contents
Facts about Proteasome subunit beta type-1.
Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required.
Human | |
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Gene Name: | PSMB1 |
Uniprot: | P20618 |
Entrez: | 5689 |
Belongs to: |
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peptidase T1B family |
EC 3.4.25.1; FLJ25321; HC5; KIAA1838; Macropain subunit C5; Multicatalytic endopeptidase complex subunit C5; PMSB1; proteasome (prosome, macropain) subunit, beta type, 1; proteasome beta 1 subunit; Proteasome component C5; Proteasome gamma chain; proteasome subunit beta type-1; proteasome subunit HC5; PSC5
Mass (kDA):
26.489 kDA
Human | |
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Location: | 6q27 |
Sequence: | 6; NC_000006.12 (170535120..170553307, complement) |
Cytoplasm. Nucleus.
In this article, we'll discuss the PSMB1 marker and its detecting methods. We will also discuss how immunohistochemistry may be of benefit to researchers who use this marker. For more information, read on to discover the best applications for this marker in immunohistochemistry. This article was written with an end user in mind. We'll cover the application in immunohistochemistry, detection methods, and more.
If you're looking for the best PSMB1 marker, look no further than Boster Bio. They offer highly-affinity primary antibodies tested in immunohistochemistry and Western Blotting. These antibodies have been cited more than 25 times over the years and are accepted for use in the scientific community. To learn more on PSMB1 antibody for research purposes, visit Boster Bio's website.
The PSMB1 Marker can be used to examine the functions of proteasomes. It degrades intracellular protein poly-ubiquitinated. It is believed to regulate inflammatory reactions and signal transduction. Its dysregulation is also implicated with the pathogenesis in neurodegenerative, autoimmune, and autoimmune diseases. PSMB1 is an active site in the 20S proteaseasome. It is still not clear what the role of PSMB1 subunits will be.
PSMB1 negatively affects innate immune responses to RNA viruses. PSMB1 actually inhibits IFNb/ISR-e. It also targets IKKe. These proteins are critical components of innate immune signaling. PSMB1 plays an essential role in the regulation or innate immune reactions. The PSMB1 markers are not used for this purpose.
The detection methods of PSMB1 markers are based on antibodies that recognize the ubiquitin-conjugating enzymes Ube1 and Ube2D3, which are both 25 kDa. Other markers that could be useful include the 20S core subunits PMSB1 and PMSA1-7 (a1 to a7) and UCHL1, a ubiquitin-C-terminal hydrolase. Both companies manufacture antibodies for these proteins.
The RTRT method showed a significantly lower level of Psmb1 after THP treatment, while the semi-quantitative PCR did not show significant differences in treatment days. The expression of Ube2d3 was significantly lower in the THP treatment animals' corpus epididymis, and caput. These results were consistent with the results obtained by the RT-RT method.
Immunohistochemistry is a technique that detects antigens and haptens from tissue. Different techniques can be used to visualize antigen specificity and localization. These techniques are widely used in pathology, and are a critical part of the diagnostic process for cancer. Different cancers may express specific antigens either de novo or up-regulated. This article will discuss IHC's many applications and its importance in diagnostic laboratories.
One of most challenging applications in immunohistochemistry is microarrays from fixed tissues. These microarrays enable researchers to simultaneously examine proteins from thousands of samples. One such sample is the esophagus, which may contain hundreds of thousands of inflammatory cells. The esophagus can also be used to apply immunohistochemistry.
PMID: 2025653 by Tamura T., et al. Molecular cloning and sequence analysis of cDNAs for five major subunits of human proteasomes (multi-catalytic proteinase complexes).
PMID: 2306472 by Lee L.W., et al. Relationships among the subunits of the high molecular weight proteinase, macropain (proteasome).