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Product Info Summary
| SKU: | PB10090 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PSMA4 Antibody Picoband®
SKU/Catalog Number
PB10090
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PSMA4 Antibody Picoband® catalog # PB10090. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PSMA4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10090)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human PSMA4, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10090 is reactive to PSMA4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated molecular weight
29484 MW
Background of PSMA4
Proteasome subunit alpha type-4, also known as macropain subunit C9, proteasome component C9, and 20S proteasome subunit alpha-3, is a protein that in humans is encoded by the PSMA4 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10090 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat liver tissue, mouse spleen tissue, HELA whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PSMA4 using anti-PSMA4 antibody (PB10090).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA4 antigen affinity purified polyclonal antibody (Catalog # PB10090) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMA4 at approximately 29 kDa. The expected band size for PSMA4 is at 29 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PSMA4 (Source: Uniprot.org, NCBI)
Gene Name
PSMA4
Full Name
Proteasome subunit alpha type-4
Weight
29484 MW
Superfamily
peptidase T1A family
Alternative Names
Proteasome subunit alpha type-4;3.4.25.1;Macropain subunit C9;Multicatalytic endopeptidase complex subunit C9;Proteasome component C9;Proteasome subunit L;PSMA4;HC9, PSC9; PSMA4 HC9, HsT17706, PSC9 proteasome 20S subunit alpha 4 proteasome subunit alpha type-4|macropain subunit C9|multicatalytic endopeptidase complex subunit C9|proteasome (prosome, macropain) subunit, alpha type, 4|proteasome component C9|proteasome subunit HC9|proteasome subunit L|proteasome subunit alpha 4|proteasome subunit alpha3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PSMA4, check out the PSMA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PSMA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PSMA4 Antibody Picoband® (PB10090)
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Customer Q&As
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5 Customer Q&As for Anti-PSMA4 Antibody Picoband®
Question
We want to test anti-PSMA4 antibody PB10090 on mouse embryonic kidney for research purposes, then I may be interested in using anti-PSMA4 antibody PB10090 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
The products we sell, including anti-PSMA4 antibody PB10090, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-07
Question
Does PB10090 anti-PSMA4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Jackson
Verified customer
Asked: 2017-09-11
Answer
You can see on the product datasheet, PB10090 anti-PSMA4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-11
Question
We are currently using anti-PSMA4 antibody PB10090 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
J. Singh
Verified customer
Asked: 2016-10-06
Answer
The anti-PSMA4 antibody (PB10090) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-10-06
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-PSMA4 antibody PB10090. Let me know if you need anything else.
P. Banerjee
Verified customer
Asked: 2014-10-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-06
Question
I see that the anti-PSMA4 antibody PB10090 works with WB, what is the protocol used to produce the result images on the product page?
G. Anderson
Verified customer
Asked: 2014-09-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-09-04
