Product Info Summary
SKU: | PB10020 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-ABCE1 Antibody Picoband®
SKU/Catalog Number
PB10020
PB1072 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ABCE1 Antibody Picoband® catalog # PB10020. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ABCE1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10020)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ABCE1 recombinant protein (Position: K419-D599). Human ABCE1 shares 100% amino acid (aa) sequence identity with mouse ABCE1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10020 is reactive to ABCE1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
67 kDa
Calculated molecular weight
67314 MW
Background of ABCE1
ATP binding cassette E1 (ABCE1, also RNase L inhibitor) is an ATPase found in humans involved in viral assembly. It is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily. ABCE1 inhibits the action of ribonuclease L. Ribonuclease L normally binds to 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) and inhibits the interferon-regulated 2-5A/RNase L pathway, which is used by viruses. ABCE1 heterodimerize with ribonuclease L and prevents its interaction with 2-5A, antagonizing the anti-viral properties of ribonuclease L, and allow the virus to synthesize viral proteins. It has also been implicated to have an effect in tumorcell proliferation and antiapoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10020 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat spleen tissue, mouse thymus tissueHELA whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ABCE1 using anti-ABCE1 antibody (PB10020).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: mouse thymus tissue lysates.
Lane 3: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody (Catalog # PB10020) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCE1 at approximately 67 kDa. The expected band size for ABCE1 is at 67 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ABCE1 (Source: Uniprot.org, NCBI)
Gene Name
ABCE1
Full Name
ATP-binding cassette sub-family E member 1
Weight
67314 MW
Superfamily
ABC transporter superfamily
Alternative Names
ATP-binding cassette sub-family E member 1;2'-5'-oligoadenylate-binding protein;HuHP68;RNase L inhibitor;Ribonuclease 4 inhibitor;RNS4I;ABCE1;RLI, RNASEL1, RNASELI, RNS4I;OK/SW-cl.40; ABCE1 ABC38, OABP, RLI, RLI1, RNASEL1, RNASELI, RNS4I ATP binding cassette subfamily E member 1 ATP-binding cassette sub-family E member 1|2-5-oligoadenylate-binding protein|ATP-binding cassette, sub-family E (OABP), member 1|RNase L inhibitor 1|huHP68|ribonuclease 4 inhibitor|ribonuclease L (2,5-oligoisoadenylate synthetase-dependent) inhibitor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCE1, check out the ABCE1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABCE1 Antibody Picoband® (PB10020)
Hello CJ!
No publications found for PB10020
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-ABCE1 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-ABCE1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-ABCE1 Antibody Picoband®
Question
Is this PB10020 anti-ABCE1 antibody reactive to the isotypes of ABCE1?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
The immunogen of PB10020 anti-ABCE1 antibody is E. coli-derived human ABCE1 recombinant protein (Position: K419-D599). Human ABCE1 shares 100% amino acid (aa) sequence identity with mouse ABCE1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-28
Question
I see that the anti-ABCE1 antibody PB10020 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-05
Question
We are currently using anti-ABCE1 antibody PB10020 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
The anti-ABCE1 antibody (PB10020) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-17
Question
Is a blocking peptide available for product anti-ABCE1 antibody (PB10020)?
D. Dhar
Verified customer
Asked: 2018-05-11
Answer
We do provide the blocking peptide for product anti-ABCE1 antibody (PB10020). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-05-11
Question
Do you have a BSA free version of anti-ABCE1 antibody PB10020 available?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ABCE1 antibody PB10020 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-19
Question
Does anti-ABCE1 antibody PB10020 work for WB with cervix carcinoma erythroleukemia?
T. Miller
Verified customer
Asked: 2017-02-16
Answer
According to the expression profile of cervix carcinoma erythroleukemia, ABCE1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-ABCE1 antibody PB10020 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2017-02-16
Question
See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-ABCE1 antibody PB10020. Please let me know if you require anything else.
M. Miller
Verified customer
Asked: 2015-07-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-07-23