Product Info Summary
SKU: | A01960-2 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PSIP1 Antibody Picoband™
SKU/Catalog Number
A01960-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PSIP1 Antibody Picoband™ catalog # A01960-2. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PSIP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01960-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human PSIP1 recombinant protein (Position: D280-N530).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01960-2 is reactive to PSIP1 in Human, Mouse, Rat
Applications
A01960-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
85 kDa
Calculated molecular weight
52588 MW
Background of LEDGF
PC4 and SFRS1 interacting protein 1, also known as lens epithelium-derived growth factor (LEDGF/p75), dense fine speckles 70kD protein (DFS 70) or transcriptional coactivator p75/p52, is a protein that in humans is encoded by the PSIP1 gene. PSIP1 is a chromatin associated protein that is shown to act as a transcriptional coactivator as well as an RNA-binding protein. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a01960 2 psip1 primary antibodies wb testing 1 a01960 2 psip1 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a01960-2-psip1-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of PSIP1 using anti-PSIP1 antibody (A01960-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSIP1 antigen affinity purified polyclonal antibody (Catalog # A01960-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSIP1 at approximately 85 kDa. The expected band size for PSIP1 is at 60 kDa.
![a01960 2 psip1 primary antibodies ihc testing 2 a01960 2 psip1 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a01960-2-psip1-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of PSIP1 using anti-PSIP1 antibody (A01960-2).
PSIP1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSIP1 Antibody (A01960-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a01960 2 psip1 primary antibodies ihc testing 3 a01960 2 psip1 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a01960-2-psip1-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of PSIP1 using anti-PSIP1 antibody (A01960-2).
PSIP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSIP1 Antibody (A01960-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a01960 2 psip1 primary antibodies ihc testing 4 a01960 2 psip1 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a01960-2-psip1-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of PSIP1 using anti-PSIP1 antibody (A01960-2).
PSIP1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSIP1 Antibody (A01960-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a01960 2 psip1 primary antibodies ihc testing 5 a01960 2 psip1 primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a01960-2-psip1-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of PSIP1 using anti-PSIP1 antibody (A01960-2).
PSIP1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSIP1 Antibody (A01960-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a01960 2 psip1 primary antibodies ihc testing 6 a01960 2 psip1 primary antibodies ihc testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a01960-2-psip1-primary-antibodies-ihc-testing-6.jpg)
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Figure 6. IHC analysis of PSIP1 using anti-PSIP1 antibody (A01960-2).
PSIP1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSIP1 Antibody (A01960-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a01960 2 psip1 primary antibodies ihc testing 7 a01960 2 psip1 primary antibodies ihc testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a01960-2-psip1-primary-antibodies-ihc-testing-7.jpg)
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Figure 7. IHC analysis of PSIP1 using anti-PSIP1 antibody (A01960-2).
PSIP1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSIP1 Antibody (A01960-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a01960 2 psip1 primary antibodies if testing 8 a01960 2 psip1 primary antibodies if testing 8](https://www.bosterbio.com/media/catalog/product/a/0/a01960-2-psip1-primary-antibodies-if-testing-8.jpg)
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Figure 8. IF analysis of PSIP1 using anti-PSIP1 antibody (A01960-2) and anti-Beta Tubulin antibody (M01857-3).
PSIP1 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSIP1 Antibody (A01960-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a01960 2 psip1 primary antibodies fcm testing 9 a01960 2 psip1 primary antibodies fcm testing 9](https://www.bosterbio.com/media/catalog/product/a/0/a01960-2-psip1-primary-antibodies-fcm-testing-9.png)
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Figure 9. Flow Cytometry analysis of K562 cells using anti-PSIP1 antibody (A01960-2).
Overlay histogram showing K562 cells stained with A01960-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSIP1 Antibody (A01960-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PSIP1 (Source: Uniprot.org, NCBI)
Gene Name
PSIP1
Full Name
PC4 and SFRS1-interacting protein
Weight
52588 MW
Superfamily
HDGF family
Alternative Names
CLL-associated antigen KW-7; Dense fine speckles 70 kDa protein; DFS 70; DFS70; LEDGF; LEDGFDFS 70; Lens epithelium-derived growth factor; MGC74712; p52; p75; PAIP; PC4 and SFRS1 interacting protein 1; PC4 and SFRS1 interacting protein 2; PC4 and SFRS1-interacting protein; PSIP1; PSIP2; transcriptional coactivator p52/p75; Transcriptional coactivator p75/p52 PSIP1 DFS70, LEDGF, PAIP, PSIP2, p52, p75 PC4 and SFRS1 interacting protein 1 PC4 and SFRS1-interacting protein|CLL-associated antigen KW-7|dense fine speckles 70 kDa protein|lens epithelium-derived growth factor|transcriptional coactivator p52/p75
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PSIP1, check out the PSIP1 Infographic
![PSIP1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PSIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PSIP1 Antibody Picoband™ (A01960-2)
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