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Product Info Summary
| SKU: | M02128 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PSD95 DLG4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02128
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PSD95 DLG4 Rabbit Monoclonal Antibody catalog # M02128. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PSD95 DLG4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02128)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DAF-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PSD95
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02128 is reactive to DLG4 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
95 kDa
Calculated molecular weight
80495 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02128 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20
FC 1:20
Positive Control
WB: rat brain tissue, rat C6 whole cell, mouse brain tissue
ICC/IF: U87-MG cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DLG4 using anti-DLG4 antibody (M02128).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLG4 antigen affinity purified monoclonal antibody (Catalog # M02128) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLG4 at approximately 95 kDa. The expected band size for DLG4 is at 80 kDa.
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Immunofluorescent analysis of U87-MG cells, using PSD95 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For DLG4 (Source: Uniprot.org, NCBI)
Gene Name
DLG4
Full Name
Disks large homolog 4
Weight
80495 MW
Superfamily
MAGUK family
Alternative Names
Disks large homolog 4;Postsynaptic density protein 95;PSD-95;Synapse-associated protein 90;SAP-90;SAP90;DLG4;PSD95; DLG4 MRD62, PSD95, SAP-90, SAP90 discs large MAGUK scaffold protein 4 disks large homolog 4|Tax interaction protein 15|discs large homolog 4|post-synaptic density protein 95|synapse-associated protein 90
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DLG4, check out the DLG4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DLG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PSD95 DLG4 Rabbit Monoclonal Antibody (M02128)
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