Disks large homolog 4 Antibodies

Disks large homolog 4 (DLG4)

Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling.

Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by keeping the station intracellularly.

Gene Information

Human
Gene Name:	DLG4
Uniprot:	P78352
Entrez:	1742

Family

Belongs to:
MAGUK family

Alternative Names

discs, large homolog 4 (Drosophila); disks large homolog 4; DLG4; FLJ97752; FLJ98574; Postsynaptic density protein 95; post-synaptic density protein 95; PSD95; PSD-95; PSD95SAP90SAP-90discs large homolog 4; SAP90; Synapse-associated protein 90; Tax interaction protein 15

Molecular Weight

Mass (kDA):
80.495 kDA

Genomic Context

Human
Location:	17p13.1
Sequence:	17; NC_000017.11 (7187180..7220050, complement)

Tissue Specificity

Brain.

Subcellular Localizations

Cell membrane; Lipid-anchor; Cytoplasmic side. Cell junction, synapse, postsynaptic density. Cell junction, synapse. Cytoplasm. Cell projection, axon. Cell projection, dendritic spine. Cell projection, dendrite. Cell junction, synapse, presynapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.

Diseases

• Schizophrenia
• Alzheimer's Disease
• Neoplasms
• Depressive Disorder
• Major Depressive Disorder
• Nervousness
• Bipolar Disorder
• Impaired Cognition
• Tissue Adhesions
• Dementia
• Malignant Neoplasms
• Neuropathology Disease

• Ischemia
• Cerebrovascular Accident
• Postmortem Changes
• Mental Disorders
• Neuroblastoma
• Nerve Degeneration
• Autistic Disorder

Interacting Proteins

• CRIPT
• DLGAP1
• ERBB4
• Gpsm2
• GRIN2A

• GRIN2B
• GRIN2C
• KCNA1
• Kcna1
• Kcna2

• Kcna3
• KCNA4
• Kcna4
• LRP1
• MDM2

• Neto1

Pathways

• Localization
• Pathogenesis
• Translation
• Synaptic Transmission
• Neurogenesis
• Cell Division
• Cell Adhesion
• Aging
• Brain Development
• Asymmetric Cell Division
• Cognition
• Signal Transmission
• Endocytosis

• Neuroprotection
• Cell Death
• Protein Folding
• Synapse Maturation
• Golgi Localization
• Cell Migration
• Cerebral Cortex Development

PTMs

• Phosphorylation
• Oxidation
• Dephosphorylation

• Palmitoylation
• Biotinylation
• Reduction

• Acetylation

Research Areas

• Neuronal Cell Markers
• Neuroscience
• Neurotransmission

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DLG4

What Are the Best Uses of the DLG4 Marker in Boster Bio?

Boster Bio is a great alternative if you're planning to purchase one. It is not unusual to encounter problems in an experiment. With the proper controls, you can eliminate the majority of these causes. Boster Bio optimization guides can help you decide what to do next. These guides contain important information that will help you determine which option is best for you.

Anti-PSD95/DLG4 marker

Boster Bio's anti-PSD95/DLG4 antibody is an anti-rabbit monoclonal antibody that connects to the human gene DLG4. It binds to the DLG4 protein (a component of MAGUK). This antibody has shown a broad range of activity in immunohistochemistry, IF/WB, and IP. As an anti-PSD95/DLG4 antibody, it has been shown to affect synaptic plasticity related to NMDA receptor signaling and hippocampumumumumasergic synaptic plasticity.

This research has found that clemastine, a peptide that is naturally found in the body, can affect several neurodegeneration-related proteins. Clemastine is able to reverse the down-regulation of PSD95/DLG4 and other synaptic-related proteins found in older mice. These results suggest that clemastine may improve hippocampal-dependent memory deficits.

Optimization

The study presents a number of new regulators of DLG4 Splicing. It also validates the method of identifying these regulators by analyzing libraries of cDNAsand shRNAs and small molecules. This method has the potential to enhance effectiveness in DLG4 splicing research. The study also identified a variety of new regulators that affect the expression of DLG4.

Troubleshooting

This troubleshooting guide can be used to identify the reason why your Western Blot was not able to detect DLG4 protein. If the protein marker is still visible on your membrane, then the transfer of the prestained protein marker to it should be successful. If it is not, you need to conduct further tests. If you have not succeeded in transferring DLG4 protein the membrane should be clear. The protein cannot be identified when the transfer is unsuccessful.

Applications

The DLG4 marker is a neuronal protein encoded in the DLG4 target gene. It is estimated to weigh about 80.5 kilobaltons. There are canine orthologs to the DLG4 gene. This protein has many uses. One of them is that it has been shown to reduce tumors in CaSki cells. Its regulation is influenced by cell density. This makes it a great tool to study neuronal functions.

DLG4 is an inhibitor of cervical cancer cells. Its restoration reduced the carcinogenic characteristics of CaSki cells in agarose medium. In vitro, CaSki/DLG4 cells lines were less tumorigenic than control, however, they did not show differences in morphology, exponential growth, or the morphology. These results suggest that the revival of DLG4 expression is not toxic to normal cells.

DLG4 encodes the protein that microglia that are immature can synthesize. DLG4 plays a role in the development process and in inflammation and could also function as a hub protein in microglial responses. In humans, systemic genetic variation in DLG4 has been linked to structural differences in preterm infant brains. These differences could affect the development of white matter and also interindividual vulnerability to injury.

In addition, to its ability to detect cancerous cells, DLG4 can also help identify the high-risk mucosotropic HPVs. This gene is directly linked with the E6 proteins found in the mucosotropic skins of high-risk HPVs. These results confirm the importance of DLG4 to detect cancer early. Application of the DLG4 marker to detect of cancer patients is now possible.

The DLG4 gene is expressed in cervical cancer cell lines and normal cervical Keratinocytes. The 18E6 gene is more abundant in cervical cancer cell lines than in those with DLG4. You can utilize the DLG4 gene to identify cervical cancer by checking DLG1 or DLG4 levels. DLG1 expression levels are also higher in cervical cancer cell lines with 16E6.

The DLG4 gene encodes a protein called PSD 95. The protein is scaffolding protein which is involved in crucial protein-protein interactions within the brain. It is believed to comprise of two supramodules. It plays an important part in synaptic plasticity, and also regulates changes in long-term potentiation. Its MW is due the phosphorylation process. This protein has numerous applications.