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Product Info Summary
| SKU: | A03863-3 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-PRTN3 Antibody Picoband®
View all Proteinase 3/Myeloblastin/PRTN3 Antibodies
SKU/Catalog Number
A03863-3
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PRTN3 Antibody Picoband® catalog # A03863-3. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PRTN3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03863-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PRTN3 recombinant protein (Position: A23-D241).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03863-3 is reactive to PRTN3 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
32 kDa
Calculated molecular weight
127459 MW
Background of Proteinase 3/Myeloblastin/PRTN3
Proteinase 3, also known as PRTN3, is an enzyme that in humans is encoded by the PRTN3 gene. Enables enzyme binding activity; serine-type endopeptidase activity; and signaling receptor binding activity. Involved in several processes, including mature conventional dendritic cell differentiation; membrane protein ectodomain proteolysis; and neutrophil extravasation. Located in azurophil granule lumen; cytosol; and plasma membrane raft. Colocalizes with plasma membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03863-3 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human THP-1 whole cell
IHC: human lung adenocarcinoma tissue, human tonsil tissue
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PRTN3 using anti-PRTN3 antibody (A03863-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRTN3 antigen affinity purified polyclonal antibody (Catalog # A03863-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRTN3 at approximately 32 kDa. The expected band size for PRTN3 is at 28,32 kDa.
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Figure 2. IHC analysis of PRTN3 using anti-PRTN3 antibody (A03863-3).
PRTN3 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRTN3 Antibody (A03863-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of PRTN3 using anti-PRTN3 antibody (A03863-3).
PRTN3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRTN3 Antibody (A03863-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 4. Flow Cytometry analysis of THP-1 cells using anti-PRTN3 antibody (A03863-3).
Overlay histogram showing THP-1 cells stained with A03863-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRTN3 Antibody (A03863-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PRTN3 (Source: Uniprot.org, NCBI)
Gene Name
PRTN3
Full Name
Myeloblastin
Weight
127459 MW
Superfamily
peptidase S1 family
Alternative Names
AF4/FMR2 family member 4;ALL1-fused gene from chromosome 5q31 protein;Protein AF-5q31;Major CDK9 elongation factor-associated protein;AFF4;AF5Q31, MCEF;HSPC092; PRTN3 ACPA, AGP7, C-ANCA, CANCA, MBN, MBT, NP-4, NP4, P29, PR-3, PR3 proteinase 3 myeloblastin|C-ANCA |Wegener granulomatosis auto|azurophil granule protein 7|leukocyte proteinase 3|neutrophil proteinase 4|serine proteinase, neutrophil|wegener auto
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRTN3, check out the PRTN3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRTN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRTN3 Antibody Picoband® (A03863-3)
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