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Product Info Summary
| SKU: | A05707 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Protein Wiz WIZ Antibody
SKU/Catalog Number
A05707
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein Wiz WIZ Antibody (Catalog # A05707). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
WIZ antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein Wiz WIZ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05707)
Host
Rabbit
Contents
WIZ Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
WIZ antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TFF3. The immunogen is located within the last 50 amino acids of WIZ.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of WIZ are known to exist; this antibody will detect all four.
Reactive Species
A05707 is reactive to WIZ in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
178674 MW
Background of Wiz
WIZ, also known as ZNF803, was initially identified in a mouse cerebellum cDNA library screen and its message was found to be expressed in the granule cell layers of the cerebellum as well as in the dentate gyrus and olfactory bulb. Later analysis indicates however that WIZ is ubiquitously expressed. WIZ is a nuclear protein that co-localizes with G9a, a histone methyltransferase responsible for mono- and dimethylation of H3K9 at euchromatic regions. WIZ can also associate with CtBP family proteins, known to be transcriptional co-repressors, and has been suggested to link G9a/GLP complexes to the CtBP co-repressor machinery, possibly regulating complex stability and gene silencing.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05707 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WIZ antibody can be used for detection of WIZ by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of WIZ in rat lung tissue lysate with WIZ antibody at 1 μg/mL.
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Immunohistochemistry of WIZ in human brain tissue with WIZ antibody at 2.5 μg/mL.
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Immunofluorescence of WIZ in human brain tissue with WIZ antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For WIZ (Source: Uniprot.org, NCBI)
Gene Name
WIZ
Full Name
Protein Wiz
Weight
178674 MW
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
ZNF803, ZNF803, Protein Wiz, Widely-interspaced zinc finger-containing protein Wiz|widely-interspaced zinc finger motifs|protein Wiz|widely-interspaced zinc finger-containing protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WIZ, check out the WIZ Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WIZ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein Wiz WIZ Antibody (A05707)
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