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Product Info Summary
| SKU: | A07701 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Protein Hook homolog 3 HOOK3 Antibody
SKU/Catalog Number
A07701
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein Hook homolog 3 HOOK3 Antibody (Catalog # A07701). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
HOOK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Protein Hook homolog 3 HOOK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07701)
Host
Rabbit
Contents
HOOK3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
HOOK3 antibody was raised against a 19 amino acid peptide from near the amino terminus of human HOOK3. The immunogen is located within amino acids 210 - 260 of HOOK3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
HOOK3 antibody is human, mouse and rat reactive. HOOK3 antibody is predicted to not cross-react with HOOK1 and HOOK2.
Reactive Species
A07701 is reactive to HOOK3 in Human, Mouse, Rat
Applications
A07701 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
83126 MW
Background of HOOK3
The HOOK proteins (HOOK1, HOOK2 and HOOK3) comprise a family of cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules mediating the spatial organization of diverse membrane-trafficking systems; their more divergent C-terminal domains mediate binding to organelles (1,2). HOOK3 exists as a homodimer and participates in defining the architecture and localization of the Golgi complex through its central coiled-coil domain (3). HOOK3 may regulate clearance of endocytosed receptors such as MSR1 (3,4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
HOOK3 antibody can be used for the detection of HOOK3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry at 10 μg/ml.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of HOOK3 in mouse brain tissue lysate with HOOK3 antibody at 1 μg/ml.
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Immunohistochemistry of HOOK3 in mouse brain tissue with HOOK3 antibody at 10 μg/ml.
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Immunofluorescence of HOOK3 in mouse brain tissue with HOOK3 antibody at 20 μg/ml.
Red: HOOK3 Antibody (A07701)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For HOOK3 (Source: Uniprot.org, NCBI)
Gene Name
HOOK3
Full Name
Protein Hook homolog 3
Weight
83126 MW
Superfamily
hook family
Alternative Names
FLJ31058; hHK3; HK3h-hook3; hook homolog 3 (Drosophila); protein Hook homolog 3 HOOK3 HK3 hook microtubule tethering protein 3 protein Hook homolog 3|h-hook3|hHK3|hook homolog 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HOOK3, check out the HOOK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HOOK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein Hook homolog 3 HOOK3 Antibody (A07701)
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