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Product Info Summary
| SKU: | PA1932 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Prohibitin/PHB Antibody Picoband®
View all Prohibitin Antibodies
SKU/Catalog Number
PA1932
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Prohibitin/PHB Antibody catalog # PA1932. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prohibitin/PHB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1932)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Prohibitin, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1932 is reactive to PHB in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30 kDa
Calculated molecular weight
29804 MW
Background of Prohibitin
PHB (Prohibitin), also known as PHB1, is a protein that in humans is encoded by the PHB gene. White et al. (1991) mapped the PHB gene to chromosome 17 by analysis of human-mouse somatic cell hybrid cell lines using a genomic fragment of human prohibitin DNA isolated from a library using the rat prohibitin cDNA clone. By in situ hybridization, they localized the gene to 17q21. Sato et al. (1992) isolated the human homolog of the rat prohibitin gene and mapped it to 17q12-q21 by in situ hybridization. Proliferation of tumor cells depends on new blood vessel formation (angiogenesis) that accompanies malignant progression. Anticancer therapies using angiogenesis inhibitors or cytotoxic agents targeted to the vasculature of tumors have been evaluated in clinical trials. Although white fat is a nonmalignant tissue, it has the capability to quickly proliferate and expand. Furthermore, it is highly vascularized. Rupnick et al. (2002) showed that nonspecific angiogenesis inhibitors can prevent the development of obesity of mice.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1932 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Lung Tissue, Rat Skeletal Muscle Tissue, Rat Brain Tissue, Rat Kidney Tissue, HELA Cell, MCF-7 Cell, PC-12 Cell, A549 Cell, SMMC Cell, mouse NIH3T3 whole cell
IHC: Human Lung Cancer Tissue, rat brain tissue
ICC/IF: U20S cell
ICC: HELA Cell
Validation Images & Assay Conditions
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Anti-Prohibitin antibody, PA1932, Western blotting
Lane 1: Rat Lung Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Lane 5: HELA Cell Lysate
Lane 6: MCF-7 Cell Lysate
Lane 7: PC-12 Cell Lysate
Lane 8: A549 Cell Lysate
Lane 9: SMMC Cell Lysate
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Anti-Prohibitin antibody, PA1932, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Anti-Prohibitin antibody, PA1932, ICC
ICC: HELA Cell
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Figure 4. IHC analysis of Prohibitin using anti-Prohibitin antibody (PA1932).
Prohibitin was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Prohibitin Antibody (PA1932) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 5. Western blot analysis of Prohibitin using anti-Prohibitin antibody (PA1932).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse NIH3T3 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prohibitin antigen affinity purified polyclonal antibody (Catalog # PA1932) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prohibitin at approximately 30KD. The expected band size for Prohibitin is at 30KD.
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Figure 6. IF analysis of Prohibitin using anti-Prohibitin antibody (PA1932).
Prohibitin was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Prohibitin Antibody (PA1932) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For PHB (Source: Uniprot.org, NCBI)
Gene Name
PHB
Full Name
Prohibitin
Weight
29804 MW
Superfamily
prohibitin family
Alternative Names
Prohibitin;PHB; PHB HEL-215, HEL-S-54e1, PHB prohibitin prohibitin|epididymis luminal protein 215|epididymis secretory sperm binding protein Li 54e
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PHB, check out the PHB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PHB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prohibitin/PHB Antibody Picoband® (PA1932)
Hello CJ!
PA1932 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Positional and expressive alteration of prohibitin during the induced differentiation of human hepatocarcinoma SMMC-7721 cells
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Customer Q&As
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6 Customer Q&As for Anti-Prohibitin/PHB Antibody Picoband®
Question
We are currently using anti-Prohibitin/PHB antibody PA1932 for human tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
The anti-Prohibitin/PHB antibody (PA1932) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-03
Question
I see that the anti-Prohibitin/PHB antibody PA1932 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-05
Question
Does PA1932 anti-Prohibitin/PHB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
It shows on the product datasheet, PA1932 anti-Prohibitin/PHB antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-12
Question
I have a question about product PA1932, anti-Prohibitin/PHB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-12
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1932 anti-Prohibitin/PHB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-12
Question
My question regards to test anti-Prohibitin/PHB antibody PA1932 on human brain for research purposes, then I may be interested in using anti-Prohibitin/PHB antibody PA1932 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Edwards
Verified customer
Asked: 2016-06-30
Answer
The products we sell, including anti-Prohibitin/PHB antibody PA1932, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-06-30
Question
Is this PA1932 anti-Prohibitin/PHB antibody reactive to the isotypes of PHB?
R. Krishna
Verified customer
Asked: 2013-12-05
Answer
The immunogen of PA1932 anti-Prohibitin/PHB antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Prohibitin(240-256aa KLEAAEDIAYQLSRSRN), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-12-05
