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Product Info Summary
| SKU: | PB9782 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Prolactin Receptor/PRLR Antibody Picoband®
View all Prolactin R Antibodies
SKU/Catalog Number
PB9782
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Prolactin Receptor/PRLR Antibody Picoband® catalog # PB9782. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prolactin Receptor/PRLR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9782)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PRLR, different from the related mouse sequence by eleven amino acids, and from the related rat sequence by fourteen amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9782 is reactive to PRLR in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65, 95 kDa
Calculated molecular weight
69506 MW
Background of Prolactin R
PRLR (Prolactin Receptor) is a cytokine receptor. By somatic cell hybrid analysis and by in situ hybridization, Arden et al. (1989, 1990) demonstrated that the prolactin receptor gene resides in the same chromosomal region as the growth hormone receptor gene, which has been mapped to 5p13-p12. Cunningham et al. (1990) demonstrated that zinc greatly increases the affinity of GH for the extracellular binding domain of PRLR, although it is not required for binding of GH to the growth hormone receptor or for binding of prolactin to the prolactin receptor. By mutational analysis, they showed that a cluster of 3 residues (histidine-18, histidine-21, and glutamic acid-174) in GH and histidine-188 in PRLR (conserved in all PRL receptors but not GH receptors) are likely zinc-ion ligands.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9782 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human MCF-7 whole cell lysates
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PRLR using anti-PRLR antibody (PB9782).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRLR antigen affinity purified polyclonal antibody (Catalog # PB9782) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRLR at approximately 65, 95 kDa. The expected band size for PRLR is at 70 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PRLR (Source: Uniprot.org, NCBI)
Gene Name
PRLR
Full Name
Prolactin receptor
Weight
69506 MW
Superfamily
type I cytokine receptor family
Alternative Names
Prolactin receptor;PRL-R;PRLR; PRLR HPRL, MFAB, RI-PRLR, hPRLrI prolactin receptor prolactin receptor|hPRL receptor|secreted prolactin binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRLR, check out the PRLR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRLR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prolactin Receptor/PRLR Antibody Picoband® (PB9782)
Hello CJ!
PB9782 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression
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Customer Q&As
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1 Customer Q&As for Anti-Prolactin Receptor/PRLR Antibody Picoband®
Question
We are currently using anti-Prolactin Receptor/PRLR antibody PB9782 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
The anti-Prolactin Receptor/PRLR antibody (PB9782) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-06
