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Product Info Summary
| SKU: | A14637 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Prickle-like protein 4 PRICKLE4 Antibody
SKU/Catalog Number
A14637
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Prickle-like protein 4 PRICKLE4 Antibody (Catalog # A14637). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
PRICKLE4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Prickle-like protein 4 PRICKLE4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14637)
Host
Rabbit
Contents
PRICKLE4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PRICKLE4 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PRICKLE4. The immunogen is located within the first 50 amino acids of PRICKLE4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Two isoforms of PRICKLE4 are known to exist. PRICKLE4 antibody is predicted to not cross-react with other PRICKLE protein family members.
Reactive Species
A14637 is reactive to PRICKLE4 in Human
Applications
A14637 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
37551 MW
Background of PRICKLE4
PRICKLE4, also known as over-expressed breast tumor protein (OBTP), is a member of the LIM family of proteins. It possesses two LIM domains as well as a PET domain and is thought to localize to the nucleus. Its expression in multiple malignant tissue differentiations indicate that it may play a role in cancer differentiation. Two other members of the LIM family, PRICKLE1 and PRICKLE2, are thought to function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity, suggesting the PRICKLE4 may play a similar role.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PRICKLE4 antibody can be used for detection of PRICKLE4 by Western blot at 0.25 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PRICKLE4 in A549 cell lysate with PRICKLE4 antibody at 0.25 μg/ml in the (A) absence and (B) presence of blocking peptide.
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Immunocytochemistry of PRICKLE4 in A549 cells with PRICKLE4 antibody at 5 μg/mL.
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Immunofluorescence of PRICKL4 in A549 cells with PRICKLE4 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For PRICKLE4 (Source: Uniprot.org, NCBI)
Gene Name
PRICKLE4
Full Name
Prickle-like protein 4
Weight
37551 MW
Superfamily
prickle / espinas / testin family
Alternative Names
over-expressed breast tumor protein; prickle homolog 4 (Drosophila); prickle-like protein 4 PRICKLE4 C6orf49, OBTP, OEBT, TOMM6 prickle planar cell polarity protein 4 prickle-like protein 4|over-expressed breast tumor protein|overexpressed breast tumor protein|overexpressed breast tumour protein|prickle homolog 4|translocase of outer mitochondrial membrane 6 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRICKLE4, check out the PRICKLE4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRICKLE4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prickle-like protein 4 PRICKLE4 Antibody (A14637)
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