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Product Info Summary
| SKU: | A05038 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Prickle-like protein 1 PRICKLE1 Antibody
SKU/Catalog Number
A05038
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Prickle-like protein 1 PRICKLE1 Antibody (Catalog # A05038). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PRICKLE1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Prickle-like protein 1 PRICKLE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05038)
Host
Rabbit
Contents
PRICKLE1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PRICKLE1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PRICKLE1. The immunogen is located within the last 50 amino acids of PRICKLE1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Multiple isoforms of PRICKLE1 are known to exist. PRICKLE1 antibody is predicted to not cross-react with other PRICKLE protein family members.
Reactive Species
A05038 is reactive to PRICKLE1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
94300 MW
Background of Prickle1
PRICKLE1, also known as RILP or EPM1B, is a Disheveled-associated protein that serves as a nuclear translocation receptor for REST/NRSF and REST4 (1, 2). It contains three N-terminal LIM domains and three C-terminal nuclear localization signals. It localizes to the cytoplasm, as well as to the nuclear membrane and expressed at higher levels in placenta. PRICKLE1 is a negative regulator of the Wnt / beta-catenin signaling pathway and is a putative tumor suppressor in human HCCs. Defects in the gene encoding PRICKLE1 are associated with autosomal recessive progressive myoclonic epilepsy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05038 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PRICKLE1 antibody can be used for detection of PRICKLE1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PRICKLE1 in human bladder tissue lysate with PRICKLE1 antibody at 1 μg/mL.
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Immunofluorescence of PRICKLE1 in human bladder cells with PRICKLE1 antibody at 20 μg/mL.
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Immunofluorescence of PRICKLE1 in Human Bladder tissue with PRICKLE1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For PRICKLE1 (Source: Uniprot.org, NCBI)
Gene Name
PRICKLE1
Full Name
Prickle-like protein 1
Weight
94300 MW
Superfamily
prickle / espinas / testin family
Alternative Names
RILP, EPM1B, RILP, Prickle-like protein 1 Prickle1|1110058P22Rik, AW215793, Prickle, b2b019C, b2b019Clo, mpk, mpk1|prickle planar cell polarity protein 1|prickle-like protein 1|prickle homolog 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRICKLE1, check out the PRICKLE1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRICKLE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prickle-like protein 1 PRICKLE1 Antibody (A05038)
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