Product Info Summary
SKU: | PA2285 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-SOX7 Antibody Picoband®
SKU/Catalog Number
PA2285
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SOX7 Antibody catalog # PA2285. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SOX7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2285)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SOX7, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2285 is reactive to SOX7 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
42197 MW
Background of Sox7
SOX7 belongs to SOX gene family and SOX proteins are transcription factors with critical roles in the regulation of diverse developmental processes. This gene is mapped to 8p23.1. SOX7 gene contains at least 2 exons. In cotransfected 293 cells, Sox7 reduced Wnt (see WNT1)/beta-catenin (see CTNNB1)- stimulated transcription. SOX7 is a potent activator of FGF3 transcription. It not only plays a role in the transcriptional regulation of genes expressed in the hemogenic endothelium but also blocks further differentiation into blood precursors. And it may be required for the survival of both hematopoietic and endothelial precursors during specification.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2285 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Human Placenta Tissue, Rat Lung Tissue, Rat Testis Tissue, HELA Whole Cell, A549 Whole Cell, HEPG2 Whole Cell, SMMC Whole Cell, NEURO Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-SOX7 antibody, PA2285, All Western blotting
All lanes: Anti-SOX7(PA2285) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: Human Placenta Tissue Lysate at 40ug
Lane 3: Rat Lung Tissue Lysate at 40ug
Lane 4: Rat Testis Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: HEPG2 Whole Cell Lysate at 40ug
Lane 8: SMMC Whole Cell Lysate at 40ug
Lane 9: NEURO Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 42KD
Protein Target Info & Infographic
Gene/Protein Information For SOX7 (Source: Uniprot.org, NCBI)
Gene Name
SOX7
Full Name
Transcription factor SOX-7
Weight
42197 MW
Alternative Names
Transcription factor SOX-7;SOX7; Sox7|SRY (sex determining region Y)-box 7|transcription factor SOX-7|SRY-box containing gene 7|mSOX7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOX7, check out the SOX7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOX7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SOX7 Antibody Picoband® (PA2285)
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No publications found for PA2285
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Customer Q&As
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1 Customer Q&As for Anti-SOX7 Antibody Picoband®
Question
We are currently using anti-SOX7 antibody PA2285 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
P. Huang
Verified customer
Asked: 2014-09-05
Answer
The anti-SOX7 antibody (PA2285) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-09-05