Product Info Summary
SKU: | A03098-2 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-PREX1 Antibody Picoband®
SKU/Catalog Number
A03098-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PREX1 Antibody Picoband® catalog # A03098-2. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PREX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03098-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PREX1 recombinant protein (Position: N643-D1155).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03098-2 is reactive to PREX1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
186 kDa
Calculated molecular weight
64406 MW
Background of P-Rex1
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein is a protein that in humans is encoded by the PREX1 gene. The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03098-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg /1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human MCF-7 whole cell
FCM: MCF-7 cell
Validation Images & Assay Conditions
![a03098 2 prex1 primary antibodies wb testing 1 a03098 2 prex1 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a03098-2-prex1-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of PREX1 using anti-PREX1 antibody (A03098-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PREX1 antigen affinity purified polyclonal antibody (Catalog # A03098-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PREX1 at approximately 186 kDa. The expected band size for PREX1 is at 186 kDa.
![a03098 2 prex1 primary antibodies fcm testing 2 a03098 2 prex1 primary antibodies fcm testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a03098-2-prex1-primary-antibodies-fcm-testing-2.png)
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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-PREX1 antibody (A03098-2).
Overlay histogram showing MCF-7 cells stained with A03098-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PREX1 Antibody (A03098-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PREX1 (Source: Uniprot.org, NCBI)
Gene Name
PREX1
Full Name
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
Weight
64406 MW
Alternative Names
NADPH oxidase 5; NOX5 PREX1 P-REX1 phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1 phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein|PIP3 dependent Rac exchange factor 1|ptdIns(3,4,5)-dependent Rac exchanger 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PREX1, check out the PREX1 Infographic
![PREX1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PREX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PREX1 Antibody Picoband® (A03098-2)
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