Anti-NADPH oxidase 5 NOX5 Antibody Picoband®

Product Name

Anti-NADPH oxidase 5 NOX5 Antibody Picoband®

View all NOX5 Antibodies

SKU/Catalog Number

PA1947

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NADPH oxidase 5 NOX5 Antibody catalog # PA1947. Tested in IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NADPH oxidase 5 NOX5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1947)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human NOX5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1947 is reactive to NOX5 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

86 kDa

Calculated molecular weight

86439 MW

Background of NOX5

NOX5 (Nadph Oxidase 5), also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to chromosome 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. Banfi et al. (2001) found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, Kamiguti et al. (2005) identified NOX5 as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells (HC), but not normal B cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA1947 is guaranteed for IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: 22RV1 Whole Cell, PANC Whole Cell, HELA Whole Cell, SKOV Whole Cell
IHC: Human Mammary Cancer Tissue
ICC: HELA Cell

Validation Images & Assay Conditions

Gene/Protein Information For NOX5 (Source: Uniprot.org, NCBI)

Alternative Names

NADPH oxidase 5;1.6.3.-;NOX5; NOX5 NADPH oxidase 5 NADPH oxidase 5|NADPH oxidase, EF-hand calcium binding domain 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis