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Product Info Summary
| SKU: | M05756 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-PPP2R1B Antibody
SKU/Catalog Number
M05756
Size
80 µl
Description
Boster Bio Anti-PPP2R1B Antibody (Catalog # M05756). Tested in WB, Flow Cytometry, IHC-P, IF application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PPP2R1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05756)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1496CT356.164.25.226
Isotype
IgG1,Κ
Immunogen
This PPP2R1B antibody is generated from a mouse immunized with a recombinant protein of human PPP2R1B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05756 is reactive to PPP2R1B in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
66214 Da
Background of PPP2R1B
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05756 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:4000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma cell line) cells labeling PPP2R1B with M05756 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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All lanes : Anti-PPP2R1B Antibody at 1:4000 dilution
Lane 1: Jurkat whole cell lysates
Lane 2: human brain lysates
Lane 3: mouse brain lysates
Lane 4: mouse lung lysates
Lane 5: rat brain lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 66 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M05756 staining PPP2R1B in human spleen sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Overlay histogram showing Jurkat cells stained with M05756 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M05756, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For PPP2R1B (Source: Uniprot.org, NCBI)
Gene Name
PPP2R1B
Full Name
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform
Weight
66214 Da
Superfamily
phosphatase 2A regulatory subunit A family
Alternative Names
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform, PP2A subunit A isoform PR65-beta, PP2A subunit A isoform R1-beta, PPP2R1B PPP2R1B PP2A-Abeta, PR65B protein phosphatase 2 scaffold subunit Abeta serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform|protein phosphatase 2, regulatory subunit A, beta|protein phosphatase 2, structural/regulatory subunit A, beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP2R1B, check out the PPP2R1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP2R1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPP2R1B Antibody (M05756)
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