Click image to see more details
Product Info Summary
| SKU: | A04723 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PP5 PPP5C Monoclonal Antibody
SKU/Catalog Number
A04723
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PP5 PPP5C Monoclonal Antibody catalog # A04723. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PP5 PPP5C Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04723)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Isotype
IgG
Immunogen
Purified recombinant human PP5 protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04723 is reactive to PPP5C in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
56879 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04723 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis using PP5 Monoclonal antibody against HeLa, Jurkat, 293T, A431, MCF7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PPP5C (Source: Uniprot.org, NCBI)
Gene Name
PPP5C
Full Name
Serine/threonine-protein phosphatase 5
Weight
56879 MW
Superfamily
PPP phosphatase family
Alternative Names
Serine/threonine-protein phosphatase 5;PP5;3.1.3.16;Protein phosphatase T;PP-T;PPT;PPP5C;PPP5; PPP5C PP5, PPP5, PPT protein phosphatase 5 catalytic subunit serine/threonine-protein phosphatase 5|PP-T|protein phosphatase T
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP5C, check out the PPP5C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP5C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PP5 PPP5C Monoclonal Antibody (A04723)
Hello CJ!
No publications found for A04723
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PP5 PPP5C Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-PP5 PPP5C Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
12 Customer Q&As for Anti-PP5 PPP5C Monoclonal Antibody
Question
Is there a BSA free version of anti-PP5 Monoclonal antibody A04723 available?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PP5 Monoclonal antibody A04723 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-10
Question
We are interested in to test anti-PP5 Monoclonal antibody A04723 on mouse frontal cortex for research purposes, then I may be interested in using anti-PP5 Monoclonal antibody A04723 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
The products we sell, including anti-PP5 Monoclonal antibody A04723, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for frontal cortex using anti-PP5 Monoclonal antibody A04723. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-07
Question
Does anti-PP5 Monoclonal antibody A04723 work for WB with frontal cortex?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
According to the expression profile of frontal cortex, PPP5C is highly expressed in frontal cortex. So, it is likely that anti-PP5 Monoclonal antibody A04723 will work for WB with frontal cortex.
Boster Scientific Support
Answered: 2019-06-21
Question
My question regarding product A04723, anti-PP5 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04723 anti-PP5 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-01
Question
Will anti-PP5 Monoclonal antibody A04723 work on feline WB with frontal cortex?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
Our lab technicians have not validated anti-PP5 Monoclonal antibody A04723 on feline. You can run a BLAST between feline and the immunogen sequence of anti-PP5 Monoclonal antibody A04723 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline frontal cortex in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-30
Question
Is this A04723 anti-PP5 Monoclonal antibody reactive to the isotypes of PPP5C?
C. Mitchell
Verified customer
Asked: 2018-02-16
Answer
The immunogen of A04723 anti-PP5 Monoclonal antibody is Purified recombinant human PP5 protein fragments expressed in E.coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-02-16
Question
Is a blocking peptide available for product anti-PP5 Monoclonal antibody (A04723)?
D. Li
Verified customer
Asked: 2018-01-19
Answer
We do provide the blocking peptide for product anti-PP5 Monoclonal antibody (A04723). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-19
Question
I have attached the WB image, lot number and protocol we used for frontal cortex using anti-PP5 Monoclonal antibody A04723. Please let me know if you require anything else.
G. Lewis
Verified customer
Asked: 2017-12-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-21
Question
I see that the anti-PP5 Monoclonal antibody A04723 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-05-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-05-25
Question
I was wanting to use your anti-PP5 Monoclonal antibody for WB for mouse frontal cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse frontal cortex identification?
E. Baker
Verified customer
Asked: 2016-04-25
Answer
It shows on the product datasheet, A04723 anti-PP5 Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse frontal cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-04-25
Question
Does A04723 anti-PP5 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Carter
Verified customer
Asked: 2016-04-01
Answer
You can see on the product datasheet, A04723 anti-PP5 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-04-01
