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Product Info Summary
| SKU: | A04783 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-POU3F2 Antibody
View all POU3F2/OCT7 Antibodies
SKU/Catalog Number
A04783
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-POU3F2 Antibody (Catalog # A04783). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
POU3F2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-POU3F2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04783)
Host
Rabbit
Contents
POU3F2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal POU3F2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human POU3F2. The immunogen is located within the last 50 amino acids of POU3F2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms are known to exist. This antibody will recognize both isoforms. POU3F2 antibody is predicted to not cross-react with other members of the POU domain class 3 family.
Reactive Species
A04783 is reactive to POU3F2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
46893 MW
Background of POU3F2/OCT7
POU3F2, also commonly known as brain-2, is a member of a family of POU domain genes expressed in mouse brain and is thought to be involved in the development of the neocortex and establishment of neural cell lineage. Recent studies suggest that POU3F2 may be involved with the development of neurodegenerative diseases as well as tumor development and proliferation. Along with the neural-lineage-specific transcription factors ASCL1 and MYT1L, POU3F2 can convert fibroblasts to functional neurons in vitro, a form of artificial stem cells termed induced neuronal (iN) cells, suggesting that these cells may be useful in the treatment of neurodegenerative diseases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04783 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
POU3F2 antibody can be used for detection of POU3F2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of POU3F2 in 3T3 cell lysate with POU3F2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of POU3F2 in human brain tissue with POU3F2 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For POU3F2 (Source: Uniprot.org, NCBI)
Gene Name
POU3F2
Full Name
POU domain, class 3, transcription factor 2
Weight
46893 MW
Superfamily
POU transcription factor family
Alternative Names
BRN2, OCT7, OTF7, OTF-7, POUF3, brn-2, oct-7, N-Oct3, BRN2, POU domain, class 3, transcription factor 2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Brain-2 POU3F2 BRN2, N-Oct3, OCT7, OTF-7, OTF7, POUF3, brn-2, oct-7 POU class 3 homeobox 2 POU domain, class 3, transcription factor 2|brain-2|brain-specific homeobox/POU domain protein 2|nervous system-specific octamer-binding transcription factor N-Oct-3|octamer-binding protein 7|octamer-binding transcription factor 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POU3F2, check out the POU3F2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POU3F2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POU3F2 Antibody (A04783)
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