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Product Info Summary
| SKU: | PB9779 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PON2 Antibody Picoband®
SKU/Catalog Number
PB9779
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PON2 Antibody Picoband® catalog # PB9779. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PON2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9779)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PON2, different from the related mouse and rat sequences by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9779 is reactive to PON2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
39397 MW
Background of PON2
PON2 is an enzyme that in humans is encoded by the PON2 gene. It is mapped to 7q21.3. This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones and important bacterial quorum-sensing mediators suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9779 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PON2 using anti-PON2 antibody (PB9779).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: HELA Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PON2 antigen affinity purified polyclonal antibody (Catalog # PB9779) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PON2 at approximately 39 kDa. The expected band size for PON2 is at 39 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PON2 (Source: Uniprot.org, NCBI)
Gene Name
PON2
Full Name
Serum paraoxonase/arylesterase 2
Weight
39397 MW
Superfamily
paraoxonase family
Alternative Names
Serum paraoxonase/arylesterase 2;PON 2;3.1.1.2;3.1.1.81;Aromatic esterase 2;A-esterase 2;Serum aryldialkylphosphatase 2;PON2; PON2 paraoxonase 2 serum paraoxonase/arylesterase 2|A-esterase 2|PON 2|aromatic esterase 2|arylesterase 2|paraoxonase nirs|serum aryldialkylphosphatase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PON2, check out the PON2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PON2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PON2 Antibody Picoband® (PB9779)
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Customer Q&As
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5 Customer Q&As for Anti-PON2 Antibody Picoband®
Question
We are currently using anti-PON2 antibody PB9779 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
The anti-PON2 antibody (PB9779) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-13
Question
Will anti-PON2 antibody PB9779 work on goat WB with skin?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
Our lab technicians have not tested anti-PON2 antibody PB9779 on goat. You can run a BLAST between goat and the immunogen sequence of anti-PON2 antibody PB9779 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat skin in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-30
Question
Does PB9779 anti-PON2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Carter
Verified customer
Asked: 2018-08-29
Answer
You can see on the product datasheet, PB9779 anti-PON2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-29
Question
My question regarding product PB9779, anti-PON2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9779 anti-PON2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-10
Question
Is there a BSA free version of anti-PON2 antibody PB9779 available?
M. Li
Verified customer
Asked: 2014-09-15
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PON2 antibody PB9779 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-09-15
