PON1 Antibodies

Serum paraoxonase/arylesterase 1 (PON1)

Hydrolyzes the toxic metabolites of an assortment of organophosphorus insecticides. Capable of hydrolyzing a wide spectrum of organophosphate substrates and lactones, and a range of aromatic carboxylic acid esters.

Mediates an enzymatic defense of low density lipoproteins against oxidative modification and the consequent series of events resulting in atheroma formation. .

Gene Information

Human
Gene Name:	PON1
Uniprot:	P27169
Entrez:	5444

Family

Belongs to:
paraoxonase family

Alternative Names

A-esterase 1; Aromatic esterase 1; arylesterase B-type; EC 3.1.1.2; EC 3.1.1.81; EC 3.1.8.1; ESA; ESAesterase A; K-45; MVCD5; paraoxonase 1; PON 1; PON1; PONparaoxonase B-type; serum aryldiakylphosphatase; Serum aryldialkylphosphatase 1; serum paraoxonase/arylesterase 1

Molecular Weight

Mass (kDA):
39.731 kDA

Genomic Context

Human
Location:	7q21.3
Sequence:	7; NC_000007.14 (95297676..95324532, complement)

Tissue Specificity

Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.

Subcellular Localizations

Secreted, extracellular space.

Diseases

• Atherosclerosis
• Cardiovascular Diseases
• Diabetes Mellitus
• Coronary Heart Disease
• Coronary Artery Disease
• Heart Diseases
• Inflammation
• Diabetes Mellitus, Non-insulin-dependent
• Arteriosclerosis
• Infarction
• Hypertensive Disease
• Myocardial Infarction

• Poisoning
• Obesity
• Cerebrovascular Accident
• Malignant Neoplasms
• Kidney Failure, Chronic
• Kidney Diseases
• Vascular Diseases

Pathways

• Pathogenesis
• Aging
• Lipoprotein Oxidation
• Cholesterol Efflux
• Transport
• Lipid Oxidation
• Secretion
• Cholesterol Transport
• Inflammatory Response
• Reverse Cholesterol Transport
• Excretion
• Localization
• Quorum Sensing

• Coagulation
• Platelet Aggregation
• Menopause
• Virulence
• Glycosylation
• Protein Oxidation
• Glomerular Filtration

PTMs

• Oxidation
• Phosphorylation
• Cleavage
• Glycosylation
• Reduction
• Cysteinylation

• Nitration
• Acetylation
• Glutathionylation
• Phosphorothioation
• Acylation
• Methylation

• Myristoylation

Research Areas

• Alzheimers Research
• Cancer
• Lipid and Metabolism
• Neurodegeneration
• Neuroscience

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PON1

Boster Bio: Best Uses of the PON1 Marker

Boster Bio: Best Uses for the PON1 marker was developed specifically for cell biology research. Its extremely specific antibody has a high affinity for PON1 as with high lysate quality when combined with beads that bind to protein A/G. Here are three strategies to optimize your experiments with the PON1 marker. Read on to learn more. But first, let's get a better understanding of the PON1 marker itself.

Techniques in molecular biology

There are numerous advantages when using different molecular biological methods to identify the PON1 mark. These methods use different DNA sequences to construct phylogenetic trees. Each method has its pros and disadvantages. Here are some examples of the various techniques used for determining the PON1 marker. It is important to know how each works before using it in your research. Here are a few of the most popular methods:

Plasmids are artificial vectors for cloning, which contain cos genes from phage lambda. These DNA molecules aren't part of the normal genome of bacterial organisms. They do not require cells to live in non-selective environments. Moreover, some of them can be integrated into the host genome. Plasmids can also serve as cloning vectors. These techniques can be utilized in molecular science to determine the function of gene or protein.

To determine the level of activity in DNA and serum samples, molecular biology techniques are used to determine the PON1 marker's activity. The Cleveland Clinic study examined the relationship between systemic PON1 activity measures and prevalent CVD or future major adverse cardiovascular events. The investigators also measured the levels of several structural fatty acids oxidation products. 150 patients shared the same PON1 genotype. They looked at the two groups to determine which had the highest PON1 activity.

Utilizing these new markers, researchers can track the development and maturation of secretory cells in the development and healing lung. They can also find out if there are any abnormalities in the process of remodeling lung tissue. Further refinement of methods for expressing genes will allow identification of genes that are restricted to the bronchiolar stem cells. These techniques will enable new discoveries about the biological functions and the function of the PON1 genes. PON1 is not a specific gene found in the lung.

Researchers can monitor epithelial cell's differentiation following naphthalene exposure by using secretory markers. Western blot analysis of the abundance of mRNA in lung tissue using a PON1 primer set has revealed that the mRNA levels of Pon1, Fmo3, Cldn10 and Aldehyde Oxidase 3 increased in number after exposure to naphthalene. These tissues show 46% higher level than their steady-state levels.

Primary antibodies with high-affinity

The humoral immune system (HA) produces primary antibodies with high affinity. These antibodies recognize almost any antigen. They are generated in large repertoires and selectively selected. This method can be used to find specific HA-binding proteins in many diseases. High-affinity antibodies can be utilized in clinical trials as an effective method to identify candidates for vaccines. We will now discuss the process of affinity maturation and its application to the production of HA-specific antibody.

Next-generation sequencing can reconstruct the antibody clonal linesages, including the sequence of germ line progenitors. The sequence of the germ line can differ from that of the ancestral gene that is not mutated. Although mutations in the VL and VH gene segments can be easily identified, the original sequences VLJL or VHDJH remain undiscovered. Affinity maturation isn't an exact process, therefore it is hard to determine the exact sequences of insertions and deletions.

PON1-targeted antibodies have additional interactions with the antigen, in addition to their roles in affinity maturation. CH58, for instance, is a part of the CH58 antibody, for instance is in contact with the V2 peptide of HIV-1 envelope glycoprotein. It has the accession code 4RIS. The V2 peptide consists of an amino acid known as Lys169 that is subject to vaccination-induced immune pressure. A germ line precursor to CH58 antibody was found in both bound and unliganded V2 peptide structures. These two salt bridges are responsible to affinity maturation.

A different method is employed to determine the KD value for monoclonal antibodies. The KD value of an antibody is determined by the ratio between its on-rate and its off-rate. This ratio shows how fast the antibody binds to the target. The lower the KD value, the higher the affinity of the antibody. The next step is to select an appropriate ligand after determining the affinity.

Human IgG is transfected with the chimeric HIRMAb–PON1 fusion protein to accomplish this. The PON1 fusion proteins are then extracted using protein A affinity chromatography. The fusion protein is then bound to the heavy chain of the PON1 antibody. Then, HIRMAbPON1 binds to recombinant POON1.

High-quality lysate with protein A/G affinity beads

Boster Bio's premium lysis buffers are perfect to purify proteins and RNAs. These buffers have a high surface area, which allows antibodies to attach to beads only when they are. The high-power magnets can also aid in locating the beads and keep them out. Furthermore magnetic separation can reduce the need to centrifuge, which can cause damage to weak antigen binding and result in loss of the target proteins. Lysates of high-quality can be made in a couple of steps.

You can also utilize a buffer instead of an experiment. The lysis buffer can be utilized in the same manner as the affinity protein A/G beads. The beads can be used to lyse cells and can be reduced to desired amounts without altering the quality of the sample. Boster Bio high-quality Lysate has only the required protein.