Product Info Summary
SKU: | M00664-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse |
Host: | Mouse |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 3B11E9)
SKU/Catalog Number
M00664-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 3B11E9) catalog # M00664-3. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 3B11E9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00664-3)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
3B11E9
Isotype
Mouse IgG2b
Standard Protein
E.coli-derived human Poliovirus Receptor/PVR recombinant protein (Position: D28-E331).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00664-3 is reactive to PVR in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
70-80 kDa
Background of CD155/PVR
CD155 (cluster of differentiation 155) also known as the poliovirus receptor is a protein that in humans is encoded by the PVR gene. The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00664-3 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human A431 whole cell, human A549 whole cell, human HT1080 whole cell, human Daudi whole cell
IHC: human liver cancer tissue, mouse liver cancer tissue
FCM: PC-3 cell
Validation Images & Assay Conditions
![m00664 3 pvr primary antibodies wb testing 1 m00664 3 pvr primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m00664-3-pvr-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HT1080 whole cell lysates,
Lane 4: human Daudi whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Poliovirus Receptor/PVR antigen affinity purified monoclonal antibody (Catalog # M00664-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Poliovirus Receptor/PVR at approximately 70-80 kDa. The expected band size for Poliovirus Receptor/PVR is at 45 kDa.
![m00664 3 pvr primary antibodies ihc testing 2 m00664 3 pvr primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m00664-3-pvr-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-3).
Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
![m00664 3 pvr primary antibodies fcm testing 3 m00664 3 pvr primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m00664-3-pvr-primary-antibodies-fcm-testing-3.png)
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Figure 3. Flow Cytometry analysis of PC-3 cells using anti-Poliovirus Receptor/PVR antibody (M00664-3).
Overlay histogram showing PC-3 cells stained with M00664-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Poliovirus Receptor/PVR Antibody (M00664-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![m00664 3 pvr primary antibodies ihc testing 4 m00664 3 pvr primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/m/0/m00664-3-pvr-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-3).
Poliovirus Receptor/PVR was detected in a paraffin-embedded section of mouse liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PVR (Source: Uniprot.org, NCBI)
Gene Name
PVR
Full Name
Poliovirus receptor
Weight
Superfamily
nectin family
Alternative Names
CD155 antigen; CD155; HVED; NECL5; Necl-5; nectin-like 5; Nectin-like protein 5; poliovirus receptor; PVR; PVS; PVSFLJ25946; Tage4 PVR CD155, HVED, NECL5, Necl-5, PVS, TAGE4 PVR cell adhesion molecule poliovirus receptor|nectin-like protein 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PVR, check out the PVR Infographic
![PVR infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PVR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 3B11E9) (M00664-3)
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