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Product Info Summary
| SKU: | A00664-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Poliovirus Receptor/PVR Antibody Picoband®
SKU/Catalog Number
A00664-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Poliovirus Receptor/PVR Antibody Picoband® catalog # A00664-3. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Poliovirus Receptor/PVR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00664-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Poliovirus Receptor/PVR recombinant protein (Position: R24-R345).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00664-3 is reactive to PVR in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60-80 kDa
Calculated molecular weight
45.303kDa
Background of CD155/PVR
CD155 (cluster of differentiation 155) also known as the poliovirus receptor is a protein that in humans is encoded by the PVR gene. The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00664-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat C6 whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (A00664-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat C6 whole cell lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: mouse NIH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Poliovirus Receptor/PVR antigen affinity purified polyclonal antibody (Catalog # A00664-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Poliovirus Receptor/PVR at approximately 60-80KD. The expected band size for Poliovirus Receptor/PVR is at 60-80KD.
Protein Target Info & Infographic
Gene/Protein Information For PVR (Source: Uniprot.org, NCBI)
Gene Name
PVR
Full Name
Poliovirus receptor
Weight
45.303kDa
Superfamily
nectin family
Alternative Names
NKG2-D type II integral membrane protein; Killer cell lectin-like receptor subfamily K member 1; NK cell receptor D; NKG2-D-activating NK receptor; CD314; KLRK1; D12S2489E; NKG2D PVR CD155, HVED, NECL5, Necl-5, PVS, TAGE4 PVR cell adhesion molecule poliovirus receptor|nectin-like protein 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PVR, check out the PVR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PVR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Poliovirus Receptor/PVR Antibody Picoband® (A00664-3)
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