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Product Info Summary
| SKU: | PB9813 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DNA polymerase eta/POLH Antibody Picoband®
View all XPV/DNA polymerase eta Antibodies
SKU/Catalog Number
PB9813
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DNA polymerase eta/POLH Antibody Picoband® catalog # PB9813. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA polymerase eta/POLH Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9813)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human POLH recombinant protein (Position: A157-R361). Human POLH shares 93.6% amino acid (aa) sequence identity with mouse POLH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9813 is reactive to POLH in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
78 kDa
Calculated molecular weight
78413 MW
Background of XPV/DNA polymerase eta
DNA polymerase eta (Pol η), is a protein that in humans is encoded by the POLH gene. This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9813 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: Rat Liver Tissue, HELA Whole Cell, SW620 Whole Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of POLH using anti-POLH antibody (PB9813).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Liver Tissue Lysate at 50ug,
Lane 2: HELA Whole Cell Lysate at 40ug,
Lane 3: SW620 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLH antigen affinity purified polyclonal antibody (Catalog # PB9813) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLH at approximately 78 kDa. The expected band size for POLH is at 78 kDa.
Protein Target Info & Infographic
Gene/Protein Information For POLH (Source: Uniprot.org, NCBI)
Gene Name
POLH
Full Name
DNA polymerase eta
Weight
78413 MW
Superfamily
DNA polymerase type-Y family
Alternative Names
DNA polymerase eta;2.7.7.7;RAD30 homolog A;Xeroderma pigmentosum variant type protein;POLH;RAD30, RAD30A, XPV; POLH RAD30, RAD30A, XP-V, XPV DNA polymerase eta DNA polymerase eta|RAD30 homolog A|polymerase (DNA directed), eta|polymerase (DNA) eta|xeroderma pigmentosum variant type protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLH, check out the POLH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNA polymerase eta/POLH Antibody Picoband® (PB9813)
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Customer Q&As
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1 Customer Q&As for Anti-DNA polymerase eta/POLH Antibody Picoband®
Question
We are currently using anti-DNA polymerase eta/POLH antibody PB9813 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on primate tissues as well?
A. Lewis
Verified customer
Asked: 2014-07-22
Answer
The anti-DNA polymerase eta/POLH antibody (PB9813) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-07-22
