DNA polymerase eta Antibodies

AND POLH ELISA kits, DNA polymerase eta Proteins

Many biological assays use antibodies to detect DNA polymerase eta, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with DNA polymerase eta in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop DNA polymerase eta antibodies...

We validate the specificity of these antibodies to DNA polymerase eta by testing them on tissues known to express POLH positively and negatively. Browse below to find the POLH antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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POLH Infographic by Boster Bio

All POLH Products

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Product

Figures

Specificity

Applications

Price

Anti-DNA polymerase eta/POLH Antibody Picoband®

1 Q&As

Human, Rat

Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # PB9813
100 μg/vial

Anti-DNA polymerase eta POLH Antibody

Human, Mouse

ELISA, WB

$380
Cat # A05236
100ul

DNA polymerase eta (POLH) (NM_006502) Human Over-expression Lysate

Human

$960
Cat # LS005429
100 µg

POLH Overview

Facts about DNA polymerase eta.

POLH 3D structure. Source: alphafold

DNA polymerase eta (POLH)

DNA polymerase especially involved in the DNA repair by translesion synthesis (TLS) (PubMed:10385124, PubMed:11743006, PubMed:24449906). As a result of low processivity on both normal and damaged DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions.

Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (PubMed:24449906).

Gene Information

Human
Gene Name:	POLH
Uniprot:	Q9Y253
Entrez:	5429

Family

Belongs to:

DNA polymerase type-Y family

Alternative Names

DNA polymerase eta; DNA Polymerase, eta; EC 2.7.7.7; POLH; polymerase (DNA directed), eta; RAD30 homolog A; RAD30; RAD30A; RAD30AFLJ16395; Xeroderma pigmentosum variant type protein; XPV; XP-V; XPVFLJ21978

Molecular Weight

Mass (kDA):
78.413 kDA

Genomic Context


Human

Location:	6p21.1
Sequence:	6; NC_000006.12 (43576147..43620523)

Subcellular Localizations

Nucleus. Binding to ubiquitinated PCNA mediates colocalization to replication foci during DNA replication and persists at sites of stalled replication forks following UV irradiation (PubMed:12606586, PubMed:16357261, PubMed:24553286). After UV irradiation, recruited to DNA damage sites within 1 hour, to a maximum of about 80%; this recruitment may not be not restricted to cells active in DNA replication (PubMed:22801543). Colocalizes with TRAIP to nuclear foci (PubMed:24553286).