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Product Info Summary
| SKU: | M03720 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-POLD1 Rabbit Monoclonal Antibody
View all DNA Polymerase delta, catalytic subunit Antibodies
SKU/Catalog Number
M03720
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-POLD1 Rabbit Monoclonal Antibody catalog # M03720. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-POLD1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03720)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
21P14
Isotype
IgG
Immunogen
A synthesized peptide derived from human POLD1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03720 is reactive to POLD1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
124 kDa
Calculated molecular weight
62064 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03720 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100
Positive Control
IHC: human colon cancer, human lung adenocarcinoma tissue, human ovarian cancer tissue, human tonsil tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of DNA Polymerase delta, catalytic subunit expression in (1) Jurkat cell lysate; (2) Raw264.7 cell lysate; (3) C6 cell lysate.
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Figure 4. IHC analysis of POLD1 using anti-POLD1 antibody (M03720) .
POLD1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POLD1 Antibody (M03720) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 5. IHC analysis of POLD1 using anti-POLD1 antibody (M03720) .
POLD1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POLD1 Antibody (M03720) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 6. IHC analysis of POLD1 using anti-POLD1 antibody (M03720) .
POLD1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POLD1 Antibody (M03720) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 7. IHC analysis of POLD1 using anti-POLD1 antibody (M03720) .
POLD1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POLD1 Antibody (M03720) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For POLD1 (Source: Uniprot.org, NCBI)
Gene Name
POLD1
Full Name
DNA polymerase delta catalytic subunit
Weight
62064 MW
Superfamily
DNA polymerase type-B family
Alternative Names
Keratin, type I cytoskeletal 9;Cytokeratin-9;CK-9;Keratin-9;K9;KRT9; POLD1 CDC2, CRCS10, MDPL, POLD DNA polymerase delta 1, catalytic subunit DNA polymerase delta catalytic subunit|3-5 exodeoxyribonuclease|CDC2 homolog|DNA polymerase subunit delta p125|polymerase (DNA directed), delta 1, catalytic subunit 125kDa|polymerase (DNA) delta 1, catalytic subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLD1, check out the POLD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POLD1 Rabbit Monoclonal Antibody (M03720)
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