Anti-POFUT2 Antibody

Product Name

Anti-POFUT2 Antibody

View all POFUT2 Antibodies

SKU/Catalog Number

A08299

Size

100ul

Form

Liquid

Description

Boster Bio Anti-POFUT2 Antibody catalog # A08299. Tested in WB,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-POFUT2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08299)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human RHG04 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08299 is reactive to POFUT2 in Human, Mouse, Rat

Applications

A08299 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

49976 MW

Background of POFUT2

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Yu H., Science 279:1219-1222(1998).
Bechtel S., BMC Genomics 8:399-399(2007).
Kraft C., EMBO J. 22:6598-6609(2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:2000
ICC/IF: 1:50-1:200

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-POFUT2 Antibody A08299

Western blot analysis of extracts of various cell lines

Gene/Protein Information For POFUT2 (Source: Uniprot.org, NCBI)

Alternative Names

C21orf80; chromosome 21 open reading frame 80; FUT13EC 2.4.1.221; GDP-fucose protein O-fucosyltransferase 2; KIAA0958; O-FucT-2; Peptide-O-fucosyltransferase 2; protein O-fucosyltransferase 2 POFUT2 C21orf80, FUT13 protein O-fucosyltransferase 2 GDP-fucose protein O-fucosyltransferase 2|O-FucT-2|peptide-O-fucosyltransferase 2

For more info on POFUT2, check out the POFUT2 Infographic

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In this infographic, you will see the following information for POFUT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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