NCBP2 Colorimetric Cell-Based ELISA

Product Name

NCBP2 Colorimetric Cell-Based ELISA

See all CBP20 products

SKU/Catalog Number

EKC1739

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The NCBP2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor NCBP2 protein expression profile in cells. The kit can be used for measuring the relative amounts of NCBP2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on NCBP2.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

NCBP2 Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1739)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1739 is reactive to NCBP2 in Human, Mouse samples

Application Guarantee

EKC1739 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent	Quantity	Container
96-Well Cell Culture Clear-Bottom Microplate	1 Plates	-
10x TBS	24 ml (10x)	Clear
Quenching Buffer	24 ml (1x)	Clear
Blocking Buffer	50 ml (1x)	Clear
15x Wash Buffer	50 ml (15x)	Clear
100x Anti-NCBP2 Antibody (Rabbit Polyclonal)	60 µl (100x)	Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal)	60 µl (100x)	Green
HRP-Conjugated Anti-Rabbit IgG Antibody	6ml (1x)	Glass
HRP-Conjugated Anti-Mouse IgG Antibody	6ml (1x)	Glass
Primary Antibody Diluent	12 ml (1x)	Clear
Ready-to-Use Substrate	12 ml (1x)	Brown
Stop Solution	12 ml (1x)	Clear
Crystal Violet Solution	6ml (1x)	Glass
SDS Solution	24 ml (1x)	Clear
Adhesive Plate Seals	2 Seals	-

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
• Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
• 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
• Deionized or sterile water
• Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
• Graph paper or computer software capable of generating or displaying logarithmic functions
• Absorbent papers or vacuum aspirator
• Test tubes or microfuge tubes capable of storing 1 ml
• Orbital shaker
• Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For NCBP2 (Source: Uniprot.Org, NCBI)

Alternative Names

Nuclear cap-binding protein subunit 2;20 kDa nuclear cap-binding protein;Cell proliferation-inducing gene 55 protein;NCBP 20 kDa subunit;CBP20;NCBP-interacting protein 1;NIP1;NCBP2;CBP20;PIG55; NCBP2 CBC2, CBP20, NIP1, PIG55 nuclear cap binding protein subunit 2 nuclear cap-binding protein subunit 2|20 kDa nuclear cap-binding protein|NCBP 20 kDa subunit|NCBP interacting protein 1|cell proliferation-inducing gene 55 protein|nuclear cap binding protein subunit 2, 20kD|nuclear cap binding protein subunit 2, 20kDa

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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