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Product Info Summary
| SKU: | M00965 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-TST Antibody (Center)
SKU/Catalog Number
M00965
Size
50 µl
Description
Boster Bio Anti-TST Antibody (Center) (Catalog # M00965). Tested in WB, IHC-P application(s). This antibody reacts with Human, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TST Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00965)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This TST antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 170-203 amino acids from the Central region of human TST.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00965 is reactive to TST in Human, Rat
Reconstitution
Calculated molecular weight
33429 Da
Background of TST
Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity (By similarity). Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00965 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-TST Antibody (Center) at 1:2000 dilution
Lane 1: human liver lysate
Lane 2: Hela whole cell lysate
Lane 3: rat kidney lysate
Lane 4: rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 33 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M00965 staining TST in human kidney tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For TST (Source: Uniprot.org, NCBI)
Gene Name
TST
Full Name
Thiosulfate sulfurtransferase
Weight
33429 Da
Alternative Names
Thiosulfate sulfurtransferase, Rhodanese, TST TST RDS thiosulfate sulfurtransferase thiosulfate sulfurtransferase|epididymis secretory sperm binding protein|thiosulfate sulfurtransferase (rhodanese)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TST, check out the TST Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TST: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TST Antibody (Center) (M00965)
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