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Product Info Summary
| SKU: | A00817-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Plzf/ZBTB16 Antibody Picoband®
SKU/Catalog Number
A00817-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Plzf/ZBTB16 Antibody Picoband® catalog # A00817-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Plzf/ZBTB16 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00817-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Plzf/ZBTB16 recombinant protein (Position: K23-V673).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00817-1 is reactive to ZBTB16 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
74 kDa
Calculated molecular weight
94973 MW
Background of PLZF
Zinc finger and BTB domain-containing protein 16 is a protein that in humans is encoded by the ZBTB16 gene. This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00817-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HL-60 whole cell, human HEK293 whole cell, human THP-1 whole cell, human Hela whole cell, human Raji whole cell, human K562 whole cell, rat spleen tissue, mouse Raw2647 whole cell
ICC/IF: A431 cell
FCM: 293T cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Plzf/ZBTB1 using Plzf/ZBTB1 antibody (A00817-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human Raji whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: rat spleen tissue lysates,
Lane 8: mouse Raw264.7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plzf/ZBTB1 antigen affinity purified polyclonal antibody (Catalog # A00817-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plzf/ZBTB1 at approximately 74KD. The expected band size for Plzf/ZBTB1 is at 74KD.
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Figure 2. IF analysis of Plzf/ZBTB16 using anti-Plzf/ZBTB16 antibody (A00817-1).
Plzf/ZBTB16 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Plzf/ZBTB16 Antibody (A00817-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of 293T cells using anti-Plzf/ZBTB16 antibody (A00817-1).
Overlay histogram showing 293T cells stained with A00817-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Plzf/ZBTB16 Antibody (A00817-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ZBTB16 (Source: Uniprot.org, NCBI)
Gene Name
ZBTB16
Full Name
Zinc finger and BTB domain-containing protein 16
Weight
94973 MW
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
Fibrinogen alpha chain; FGA ZBTB16 PLZF, ZNF145 zinc finger and BTB domain containing 16 zinc finger and BTB domain-containing protein 16|promyelocytic leukaemia zinc finger|zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia)|zinc finger protein PLZF
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZBTB16, check out the ZBTB16 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZBTB16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Plzf/ZBTB16 Antibody Picoband® (A00817-1)
Hello CJ!
A00817-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Shi L,Duan Y,Yao X,Song R,Ren Y.Effects of selenium on the proliferation and apoptosis of sheep spermatogonial stem cells in vitro.Anim Reprod Sci.2020 Apr;215:106330.doi:10.1016/j.anireprosci.2020.106330.Epub 2020 Feb 22.PMID:32216931.
Species: Sheep
A00817-1 usage in article: APP:ICC, SAMPLE:SSC, DILUTION:1:100
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